ArticleProceedings of the National Academy of Sciences of the United States of America2005
Intronic regulation of matrix metalloproteinase-2 revealed by in vivo transcriptional analysis in ischemia.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 57 citations in OpenAlex.
- Direct visualization of emergent metastatic features within an ex vivo model of the tumor microenvironment.Life science alliance · 2025Article
- Dysregulated Genes, MicroRNAs, Biological Pathways, and Gastrocnemius Muscle Fiber Types Associated With Progression of Peripheral Artery Disease: A Preliminary Analysis.Journal of the American Heart Association · 2022Article
- Collateral Arteriogenesis Involves a Sympathetic Denervation That Is Associated With Abnormal α-Adrenergic Signaling and a Transient Loss of Vascular Tone.Frontiers in cardiovascular medicine · 2022Article
- FOSL2 promotes VEGF-independent angiogenesis by transcriptionnally activating Wnt5a in breast cancer-associated fibroblasts.Theranostics · 2021Article
- Tumor suppressor protein p53 negatively regulates ischemia-induced angiogenesis and arteriogenesis.Journal of vascular surgery · 2018Article
- Heat shock protein 90 inhibition by 17-DMAG attenuates abdominal aortic aneurysm formation in mice.American journal of physiology. Heart and circulatory physiology · 2015Article
- Two Distinct Isoforms of Matrix Metalloproteinase-2 Are Associated with Human Delayed Kidney Graft Function.PloS one · 2015Article
- Angiotensin II stimulates cardiac fibroblast migration via the differential regulation of matrixins and RECK.Journal of molecular and cellular cardiology · 2013Article
- Connective tissue growth factor regulates retinal neovascularization through p53 protein-dependent transactivation of the matrix metalloproteinase (MMP)-2 gene.The Journal of biological chemistry · 2012Article
- Gentamicin supplemented polyvinylidenfluoride mesh materials enhance tissue integration due to a transcriptionally reduced MMP-2 protein expression.BMC surgery · 2012Article
- A novel intracellular isoform of matrix metalloproteinase-2 induced by oxidative stress activates innate immunity.PloS one · 2012Article
- Hyperexpansion of GAA repeats affects post-initiation steps of FXN transcription in Friedreich's ataxia.Nucleic acids research · 2011Article
- Article
- Impact of gentamicin-supplemented polyvinylidenfluoride mesh materials on MMP-2 expression and tissue integration in a transgenic mice model.Langenbeck's archives of surgery · 2010Article
- An integrative genomic and proteomic approach to chemosensitivity prediction.International journal of oncology · 2009Article
- Matrix metalloproteinase control of capillary morphogenesis.Critical reviews in eukaryotic gene expression · 2008Review
- FGF-2 release from the lens capsule by MMP-2 maintains lens epithelial cell viability.Molecular biology of the cell · 2007Article
- Differential regulation of CHOP-10/GADD153 gene expression by MAPK signaling in pancreatic beta-cells.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Repeat-induced epigenetic changes in intron 1 of the frataxin gene and its consequences in Friedreich ataxia.Nucleic acids research · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Matrix metalloproteinase-2 (MMP-2) plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown. We studied the transcriptional activation of the MMP-2 gene in a model of hindlimb ischemia by using various MMP-2-lacZ reporter mice and chromatin immunoprecipitation. MMP-2 activity and mRNA were increased after hindlimb ischemia. Mice with targeted deletion of MMP-2 had impaired restoration of perfusion and a high incidence of limb gangrene, indicating that MMP-2 plays a critical role in ischemia-induced revascularization. Ischemia induced the expression and binding of c-Fos, c-Jun, JunB, FosB, and Fra2 to a noncanonical activating protein-1 (AP-1) site present in the MMP-2 promoter and decreased binding of the transcriptional repressor JunD. Ischemia also activated the expression and binding of p53 to an adjacent enhancer site (RE-1) and increased expression and binding of nuclear factor of activated T-cells-c2 to consensus sequences within the first intron. Deletion of either the 5' AP-1/RE-1 region of the promoter or substitution of the first intron abolished ischemia-induced MMP-2 transcription in vivo. Thus, AP-1 transcription factors and intronic activation by nuclear factor of activated T-cells-c2 act in concert to drive ischemia-induced MMP-2 transcription. These findings define a critical role for MMP-2 in ischemia-induced revascularization and identify both previously uncharacterized regulatory elements within the MMP-2 gene and the cognate transcription factors required for MMP-2 activation in vivo after tissue ischemia.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.