ArticleBiophysical journal2006
Sampling effects, noise, and photobleaching in temporal image correlation spectroscopy.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 79 citations in OpenAlex.
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- Bleach correction ImageJ plugin for compensating the photobleaching of time-lapse sequences.F1000Research · 2020Article
- Raster Image Correlation Spectroscopy Performance Evaluation.Biophysical journal · 2019Article
- Estimation of diffusion constants from single molecular measurement without explicit tracking.BMC systems biology · 2018Article
- Netrin-1-Regulated Distribution of UNC5B and DCC in Live Cells Revealed by TICCS.Biophysical journal · 2016Article
- An Intermittent Model for Intracellular Motions of Gold Nanostars by k-Space Scattering Image Correlation.Biophysical journal · 2015Article
- Spatial Intensity Distribution Analysis Reveals Abnormal Oligomerization of Proteins in Single Cells.Biophysical journal · 2015Article
- Exploring the dynamics of cell processes through simulations of fluorescence microscopy experiments.Biophysical journal · 2015Article
- Bayesian total internal reflection fluorescence correlation spectroscopy reveals hIAPP-induced plasma membrane domain organization in live cells.Biophysical journal · 2014Article
- Membrane orientation and lateral diffusion of BODIPY-cholesterol as a function of probe structure.Biophysical journal · 2013Article
- Development of new photon-counting detectors for single-molecule fluorescence microscopy.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Quantitative measurement of intracellular transport of nanocarriers by spatio-temporal image correlation spectroscopy.Methods and applications in fluorescence · 2013Article
- Fluorescence fluctuation approaches to the study of adhesion and signaling.Methods in enzymology · 2013Article
- Monitoring dynamic binding of chromatin proteins in vivo by fluorescence correlation spectroscopy and temporal image correlation spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2012Article
- Image correlation spectroscopy of randomly distributed disks.Journal of biological physics · 2011Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present an extensive investigation of the accuracy and precision of temporal image correlation spectroscopy (TICS). Using simulations of laser scanning microscopy image time series, we investigate the effect of spatiotemporal sampling, particle density, noise, sampling frequency, and photobleaching of fluorophores on the recovery of transport coefficients and number densities by TICS. We show that the recovery of transport coefficients is usually limited by spatial sampling, while the measurement of accurate number densities is restricted by background noise in an image series. We also demonstrate that photobleaching of the fluorophore causes a consistent overestimation of diffusion coefficients and flow rates, and a severe underestimation of number densities. We derive a bleaching correction equation that removes both of these biases when used to fit temporal autocorrelation functions, without increasing the number of fit parameters. Finally, we image the basal membrane of a CHO cell with EGFP/alpha-actinin, using two-photon microscopy, and analyze a subregion of this series using TICS and apply the bleaching correction. We show that the photobleaching correction can be determined simply by using the average image intensities from the time series, and we use the simulations to provide good estimates of the accuracy and precision of the number density and transport coefficients measured with TICS.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.