ArticleBiophysical journal2006
Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres.
Article in Biophysical journal, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 171 papers.
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Who cites it
171 citing papers in PubMed.
- Probing relaxed myosin states in hypertrophic cardiomyopathy by second harmonic-generation microscopy.eLife · 2026Article
- Polarized Light Microscopy for Quantitative Assessment of Sarcomere-Scale Structural Order in Cardiac Tissue.Journal of biophotonics · 2026Article
- Two-photon imaging microscopy of non-stained human epiretinal membranes.Biomedical optics express · 2026Article
- Double Stokes polarimetry of birefringent structures.Biomedical optics express · 2026Article
- Correlative multimodal imaging for microscale spatial mapping of collagen-gene activity interactions in human tissues.Npj imaging · 2026Article
- Second harmonic generation of myofibrils exhibits a polarization-resolved "gradient" effect.Biomedical optics express · 2025Article
- Polar organization of H&E dyes in histology tissue revealed by polarimetric nonlinear microscopy.Biomedical optics express · 2025Article
- Multiscale investigation of collagen structure in human skin and gel matrices using polarization resolved second harmonic generation microscopy.Scientific reports · 2025Article
- Multiparametric physicochemical analysis of a type 1 collagen 3D cell culture model using light and electron microscopy and mass spectrometry imaging.Scientific reports · 2025Article
- Double Stokes polarimetric microscopy for chiral fibrillar aggregates.Scientific reports · 2025Article
- Evaluation of the effects of clearing agents, fixation, and process durations on cardiovascular tissue imaging with second harmonic generation and multi-photon modalities.Frontiers in bioengineering and biotechnology · 2025Article
- Use of optical techniques to evaluate the ionizing radiation effects on biological specimens.Journal of radiation research · 2024Review
- Kinesin-14 HSET and KlpA are non-processive microtubule motors with load-dependent power strokes.Nature communications · 2024Article
- Effects of mineralization on the hierarchical organization of collagen-a synchrotron X-ray scattering and polarized second harmonic generation study.Interface focus · 2024Article
- In Situ Structural Characterization of Cardiomyocyte Microenvironment by Multimodal STED Microscopy.Photonics · 2024Article
- Spectroscopic analysis of the sum-frequency response of the carbon-hydrogen stretching modes in collagen type I.The Journal of chemical physics · 2024Article
- Failure of Autophagy in Pompe Disease.Biomolecules · 2024Review
- Analysis of age-related changes in the left ventricular myocardium with multiphoton microscopy.Biomedical optics express · 2024Article
- Diving head-first into brain intravital microscopy.Frontiers in immunology · 2024Review
- Intravital imaging of cardiac tissue utilizing tissue-stabilized heart window chamber in live animal model.European heart journal. Imaging methods and practice · 2024Article
111 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Several biologically important protein structures give rise to strong second-harmonic generation (SHG) in their native context. In addition to high-contrast optical sections of cells and tissues, SHG imaging can provide detailed structural information based on the physical constraints of the optical effect. In this study we characterize, by biochemical and optical analysis, the critical structures underlying SHG from the complex muscle sarcomere. SHG emission arises from domains of the sarcomere containing thick filaments, even within nascent sarcomeres of differentiating myocytes. SHG from isolated myofibrils is abolished by extraction of myosin, but is unaffected by removal or addition of actin filaments. Furthermore, the polarization dependence of sarcomeric SHG is not affected by either the proportion of myosin head domains or the orientation of myosin heads. By fitting SHG polarization anisotropy readings to theoretical response curves, we find an orientation for the elemental harmonophore that corresponds well to the pitch of the myosin rod alpha-helix along the thick filament axis. Taken together, these data indicate that myosin rod domains are the key structures giving SHG from striated muscle. This study should guide the interpretation of SHG contrast in images of cardiac and skeletal muscle tissue for a variety of biomedical applications.
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