ArticlePlant physiology2005
Arabidopsis vegetative storage protein is an anti-insect acid phosphatase.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
72 citing papers in PubMed, 194 citations in OpenAlex.
- The old nourishes the new: 100 years of leaf senescence studies in Plant Physiology.Plant physiology · 2026Review
- Exl1 from Pectobacterium activates a jasmonate-dependent immune network in Arabidopsis thaliana.Plant molecular biology · 2026Article
- Expression analysis of phosphatase III-B subfamily in common bean reveals two storage proteins highly expressed during seed formation and germination.Physiology and molecular biology of plants : an international journal of functional plant biology · 2026Article
- A natural variant of anProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Vacuolar phosphatases are essential for efficient nucleotide salvage in Arabidopsis.Journal of experimental botany · 2025Article
- Enhancing inositol pyrophosphate accumulation in plants alters growth, phosphate homeostasis, and insect herbivory.The Plant journal : for cell and molecular biology · 2025Article
- Isolation and Characterization of Extracellular Vesicles fromPlants (Basel, Switzerland) · 2023Article
- PRpnp, a novel dual activity PNP family protein improves plant vigour and confers multiple stress tolerance in Citrus aurantifolia.Plant biotechnology journal · 2023Article
- Proteomic Analysis of Proteins Related to Defense Responses inInternational journal of molecular sciences · 2023Article
- Diffusion and bulk flow of amino acids mediate calcium waves in plants.Science advances · 2022Article
- Micronutrients Affect Expression of Induced Resistance Genes in Hydroponically Grown Watermelon against Fusarium oxysporum f. sp. niveum and Meloidogyne incognitaPathogens (Basel, Switzerland) · 2022Article
- Metabolomic and Genomic Approach to Study Defense Induction byMetabolites · 2022Article
- Comparative Plant Transcriptome Profiling of Arabidopsis thaliana Col-0 and Camelina sativa var.Microbiology spectrum · 2022Article
- Comparison of the pathway structures influencing the temporal response of salicylate and jasmonate defence hormones inFrontiers in plant science · 2022Review
- Article
- Fine mapping of a thrips resistance QTL in Capsicum and the role of diterpene glycosides in the underlying mechanism.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Genome-wide analysis of haloacid dehalogenase genes reveals their function in phosphate starvation responses in rice.PloS one · 2021Article
- Biochemical and Molecular Characterization of PvNTD2, a Nucleotidase Highly Expressed in Nodules fromPlants (Basel, Switzerland) · 2020Article
- Targeting Nitrogen Metabolism and Transport Processes to Improve Plant Nitrogen Use Efficiency.Frontiers in plant science · 2020Review
- Proteomic Changes in the Sound Vibration-TreatedThe plant pathology journal · 2019Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Indirect evidence previously suggested that Arabidopsis (Arabidopsis thaliana) vegetative storage protein (VSP) could play a role in defense against herbivorous insects. To test this hypothesis, other AtVSP-like sequences in Arabidopsis were identified through a Basic Local Alignment Search Tool search, and their transcriptional profiles were investigated. In response to methyl jasmonate application or phosphate starvation, AtVSP and AtVSP-like genes exhibited differential expression patterns, suggesting distinct roles played by each member. Arabidopsis VSP2 (AtVSP2), a gene induced by wounding, methyl jasmonate, insect feeding, and phosphate deprivation, was selected for bacterial expression and functional characterization. The recombinant protein exhibited a divalent cation-dependent phosphatase activity in the acid pH range. When incorporated into the diets of three coleopteran and dipteran insects that have acidic gut lumen, recombinant AtVSP2 significantly delayed development of the insects and increased their mortality. To further determine the biochemical basis of the anti-insect activity of the protein, the nucleophilic aspartic acid-119 residue at the conserved DXDXT signature motif was substituted by glutamic acid via site-directed mutagenesis. This single-amino acid alteration did not compromise the protein's secondary or tertiary structure, but resulted in complete loss of its acid phosphatase activity as well as its anti-insect activity. Collectively, we conclude that AtVSP2 is an anti-insect protein and that its defense function is correlated with its acid phosphatase activity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.