ArticlePlant physiology2005
HKT1 mediates sodium uniport in roots. Pitfalls in the expression of HKT1 in yeast.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 150 citations in OpenAlex.
- Comparative analysis of HKT genes inFrontiers in plant science · 2025Article
- Structural insights into ion selectivity and transport mechanisms of Oryza sativa HKT2;1 and HKT2;2/1 transporters.Nature plants · 2024Article
- Identification of the High-Affinity Potassium Transporter Gene Family (HKT) inPlants (Basel, Switzerland) · 2023Article
- HvNCX, a prime candidate gene for the novel qualitative locus qS7.1 associated with salinity tolerance in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- A genome-wide association study identifies novel players in Na and Fe homeostasis in Arabidopsis thaliana under alkaline-salinity stress.The Plant journal : for cell and molecular biology · 2023Article
- Mechanism of high affinity potassium transporter (HKT) towards improved crop productivity in saline agricultural lands.3 Biotech · 2022Review
- Plant HKT Channels: An Updated View on Structure, Function and Gene Regulation.International journal of molecular sciences · 2021Review
- Evolution of Plant NaPlants (Basel, Switzerland) · 2021Article
- A DNA Methylation Reader-Chaperone Regulator-Transcription Factor Complex ActivatesThe Plant cell · 2020Article
- Coordinated Transport of Nitrate, Potassium, and Sodium.Frontiers in plant science · 2020Review
- Networks of Physiological Adjustments and Defenses, and Their Synergy With Sodium (NaFrontiers in plant science · 2020Article
- A cell suspension based uptake method to study high affinity glucosinolate transporters.Plant methods · 2020Article
- An extracellular cation coordination site influences ion conduction of OsHKT2;2.BMC plant biology · 2019Article
- Genome-wide identification of the HKT genes in five Rosaceae species and expression analysis of HKT genes in response to salt-stress in Fragaria vesca.Genes & genomics · 2019Article
- Role and Functional Differences of HKT1-Type Transporters in Plants under Salt Stress.International journal of molecular sciences · 2019Review
- HKT1;5 Transporter Gene Expression and Association of Amino Acid Substitutions With Salt Tolerance Across Rice Genotypes.Frontiers in plant science · 2019Article
- Pumpkin CmHKT1;1 Controls Shoot Na⁺ Accumulation via Limiting Na⁺ Transport from Rootstock to Scion in Grafted Cucumber.International journal of molecular sciences · 2018Article
- Promoter of the TmHKT1;4-A1 gene of Triticum monococcum directs stress inducible, developmental regulated and organ specific gene expression in transgenic Arbidopsis thaliana.World journal of microbiology & biotechnology · 2018Article
- The High-Affinity Potassium Transporter EpHKT1;2 From the ExtremophileFrontiers in plant science · 2018Article
- Association Analysis and Identification of ZmHKT1;5 Variation With Salt-Stress Tolerance.Frontiers in plant science · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The function of HKT1 in roots is controversial. We tackled this controversy by studying Na+ uptake in barley (Hordeum vulgare) roots, cloning the HvHKT1 gene, and expressing the HvHKT1 cDNA in yeast (Saccharomyces cerevisiae) cells. High-affinity Na+ uptake was not detected in plants growing at high K+ but appeared soon after exposing the plants to a K(+)-free medium. It was a uniport, insensitive to external K+ at the beginning of K+ starvation and inhibitable by K+ several hours later. The expression of HvHKT1 in yeast was Na+ (or K+) uniport, Na(+)-K+ symport, or a mix of both, depending on the construct from which the transporter was expressed. The Na+ uniport function was insensitive to external K+ and mimicked the Na+ uptake carried out by the roots at the beginning of K+ starvation. The K+ uniport function only took place in yeast cells that were completely K+ starved and disappeared when internal K+ increased, which makes it unlikely that HvHKT1 mediates K+ uptake in roots. Mutation of the first in-frame AUG codon of HvHKT1 to CUC changed the uniport function into symport. The expression of the symport from either mutants or constructs keeping the first in-frame AUG took place only in K(+)-starved cells, while the uniport was expressed in all conditions. We discuss here that the symport occurs only in heterologous expression. It is most likely related to the K+ inhibitable Na+ uptake process of roots that heterologous systems fail to reproduce.
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