ArticlePlant physiology2005
An egg apparatus-specific enhancer of Arabidopsis, identified by enhancer detection.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 36 citations in OpenAlex.
- Enhancers in Plant Development, Adaptation and Evolution.Plant & cell physiology · 2025Review
- Application of Single-Cell Assay for Transposase-Accessible Chromatin with High Throughput Sequencing in Plant Science: Advances, Technical Challenges, and Prospects.International journal of molecular sciences · 2024Review
- The prevalence, evolution and chromatin signatures of plant regulatory elements.Nature plants · 2019Article
- Novel mRNAs 3' end-associatedRNA (New York, N.Y.) · 2019Article
- Global Quantitative Mapping of Enhancers in Rice by STARR-seq.Genomics, proteomics & bioinformatics · 2019Article
- Patterning the Female Gametophyte of Flowering Plants.Plant physiology · 2017Review
- Genome-Wide Prediction and Validation of Intergenic Enhancers in Arabidopsis Using Open Chromatin Signatures.The Plant cell · 2015Article
- Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently generates homozygous mutants for multiple target genes in Arabidopsis in a single generation.Genome biology · 2015Article
- Transgenic manipulation of plant embryo sacs tracked through cell-type-specific fluorescent markers: cell labeling, cell ablation, and adventitious embryos.Plant reproduction · 2013Article
- pMAA-Red: a new pPZP-derived vector for fast visual screening of transgenic Arabidopsis plants at the seed stage.BMC biotechnology · 2012Article
- Nuclear behavior, cell polarity, and cell specification in the female gametophyte.Sexual plant reproduction · 2011Review
- When Double is not Twice as Much.Frontiers in plant science · 2011Article
- Article
- A versatile and reliable two-component system for tissue-specific gene induction in Arabidopsis.Plant physiology · 2006Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Despite a central role in angiosperm reproduction, few gametophyte-specific genes and promoters have been isolated, particularly for the inaccessible female gametophyte (embryo sac). Using the Ds-based enhancer-detector line ET253, we have cloned an egg apparatus-specific enhancer (EASE) from Arabidopsis (Arabidopsis thaliana). The genomic region flanking the Ds insertion site was further analyzed by examining its capability to control gusA and GFP reporter gene expression in the embryo sac in a transgenic context. Through analysis of a 5' and 3' deletion series in transgenic Arabidopsis, the sequence responsible for egg apparatus-specific expression was delineated to 77 bp. Our data showed that this enhancer is unique in the Arabidopsis genome, is conserved among different accessions, and shows an unusual pattern of sequence variation. This EASE works independently of position and orientation in Arabidopsis but is probably not associated with any nearby gene, suggesting either that it acts over a large distance or that a cryptic element was detected. Embryo-specific ablation in Arabidopsis was achieved by transactivation of a diphtheria toxin gene under the control of the EASE. The potential application of the EASE element and similar control elements as part of an open-source biotechnology toolkit for apomixis is discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.