ArticleNucleic acids research2005
SegH and Hef: two novel homing endonucleases whose genes replace the mobC and mobE genes in several T4-related phages.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 32 citations in OpenAlex.
- Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunity.Nature communications · 2026Article
- Diversity and functional characterization of HNH endonucleases encoded by lactococcalMicrobial genomics · 2025Article
- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.NAR genomics and bioinformatics · 2023Article
- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.bioRxiv : the preprint server for biology · 2023Article
- Characterization of two polyvalent phages infecting Enterobacteriaceae.Scientific reports · 2017Article
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- Comparative Functional Genomic Analysis of TwoFrontiers in microbiology · 2016Article
- Mapping homing endonuclease cleavage sites using in vitro generated protein.Methods in molecular biology (Clifton, N.J.) · 2014Article
- A bioinformatic analysis of ribonucleotide reductase genes in phage genomes and metagenomes.BMC evolutionary biology · 2013Article
- T4-related bacteriophage LIMEstone isolates for the control of soft rot on potato caused by 'Dickeya solani'.PloS one · 2012Article
- Activity, specificity and structure of I-Bth0305I: a representative of a new homing endonuclease family.Nucleic acids research · 2011Article
- Campylobacter jejuni group III phage CP81 contains many T4-like genes without belonging to the T4-type phage group: implications for the evolution of T4 phages.Journal of virology · 2011Article
- Assembly of a fragmented ribonucleotide reductase by protein interaction domains derived from a mobile genetic element.Nucleic acids research · 2011Article
- Mobile DNA elements in T4 and related phages.Virology journal · 2010Review
- Phage T4 mobE promotes trans homing of the defunct homing endonuclease I-TevIII.Nucleic acids research · 2009Article
- Biochemical and mutagenic analysis of I-CreII reveals distinct but important roles for both the H-N-H and GIY-YIG motifs.Nucleic acids research · 2009Article
- An RNA hairpin sequesters the ribosome binding site of the homing endonuclease mobE gene.Journal of bacteriology · 2009Article
- A likely pathway for formation of mobile group I introns.Current biology : CB · 2009Article
- Amino acid residues in the GIY-YIG endonuclease II of phage T4 affecting sequence recognition and binding as well as catalysis.Journal of bacteriology · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
T4 contains two groups of genes with similarity to homing endonucleases, the seg-genes (similarity to endonucleases encoded by group I introns) containing GIY-YIG motifs and the mob-genes (similarity to mobile endonucleases) containing H-N-H motifs. The four seg-genes characterized to date encode homing endonucleases with cleavage sites close to their respective gene loci while none of the mob-genes have been shown to cleave DNA. Of 18 phages screened, only T4 was found to have mobC while mobE genes were found in five additional phages. Interestingly, three phages encoded a seg-like gene (hereby called segH) with a GIY-YIG motif in place of mobC. An additional phage has an unrelated gene called hef (homing endonuclease-like function) in place of the mobE gene. The gene products of both novel genes displayed homing endonuclease activity with cleavage site specificity close to their respective genes. In contrast to intron encoded homing endonucleases, both SegH and Hef can cleave their own DNA as well as DNA from phages without the genes. Both segH and mobE (and most likely hef) can home between phages in mixed infections. We discuss why it might be a selective advantage for phage freestanding homing endonucleases to cleave both HEG-containing and HEG-less genomes.
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