ArticleJournal of virology2005
Interaction between Brome mosaic virus proteins and RNAs: effects on RNA replication, protein expression, and RNA stability.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Trans-complementation of the viral movement protein mediates efficient expression of large target genes via a tobacco mosaic virus vector.Plant biotechnology journal · 2024Article
- Complete genome sequencing and construction of full-length infectious cDNA clone ofFrontiers in microbiology · 2023Article
- Host Pah1p phosphatidate phosphatase limits viral replication by regulating phospholipid synthesis.PLoS pathogens · 2018Article
- Design of virus-based nanomaterials for medicine, biotechnology, and energy.Chemical Society reviews · 2016Review
- The Effects of SELEX Conditions on the Resultant Aptamer Pools in the Selection of Aptamers Binding to Bacterial Cells.Journal of molecular evolution · 2015Article
- The tripartite virions of the brome mosaic virus have distinct physical properties that affect the timing of the infection process.Journal of virology · 2014Article
- The plant host can affect the encapsidation of brome mosaic virus (BMV) RNA: BMV virions are surprisingly heterogeneous.Journal of molecular biology · 2014Article
- Chemical reactivity of brome mosaic virus capsid protein.Journal of molecular biology · 2012Article
- An examination of the electrostatic interactions between the N-terminal tail of the Brome Mosaic Virus coat protein and encapsidated RNAs.Journal of molecular biology · 2012Article
- The subgenomic promoter of brome mosaic virus folds into a stem-loop structure capped by a pseudo-triloop that is structurally similar to the triloop of the genomic promoter.RNA (New York, N.Y.) · 2012Article
- RNA synthesis by the brome mosaic virus RNA-dependent RNA polymerase in human cells reveals requirements for de novo initiation and protein-protein interaction.Journal of virology · 2012Article
- The coat protein leads the way: an update on basic and applied studies with the Brome mosaic virus coat protein.Molecular plant pathology · 2011Review
- A simple and general method for determining the protein and nucleic acid content of viruses by UV absorbance.Virology · 2010Article
- RNA binding by the brome mosaic virus capsid protein and the regulation of viral RNA accumulation.Journal of molecular biology · 2009Article
- Brome mosaic virus capsid protein regulates accumulation of viral replication proteins by binding to the replicase assembly RNA element.RNA (New York, N.Y.) · 2009Article
- Insights into the single-cell reproduction cycle of members of the family Bromoviridae: lessons from the use of protoplast systems.Journal of virology · 2008Review
- cis- and trans-acting functions of brome mosaic virus protein 1a in genomic RNA1 replication.Journal of virology · 2008Article
- Replication-coupled packaging mechanism in positive-strand RNA viruses: synchronized coexpression of functional multigenome RNA components of an animal and a plant virus in Nicotiana benthamiana cells by agroinfiltration.Journal of virology · 2008Article
- Nodavirus RNA replication protein a induces membrane association of genomic RNA.Journal of virology · 2007Article
- Replication-independent long-distance trafficking by viral RNAs in Nicotiana benthamiana.The Plant cell · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Brome mosaic virus (BMV) RNA replication has been examined in a number of systems, including Saccharomyces cerevisiae. We developed an efficient T-DNA-based gene delivery system using Agrobacterium tumefaciens to transiently express BMV RNAs in Nicotiana benthamiana. The expressed RNAs can systemically infect plants and provide material to extract BMV replicase that can perform template-dependent RNA-dependent RNA synthesis in vitro. We also expressed the four BMV-encoded proteins from nonreplicating RNAs and analyzed their effects on BMV RNA accumulation. The capsid protein that coinfiltrated with constructs expressing RNA1 and RNA2 suppressed minus-strand levels but increased plus-strand RNA accumulation. The replication proteins 1a and 2a could function in trans to replicate and transcribe the BMV RNAs. None of the BMV proteins or RNA could efficiently suppress posttranscriptional silencing. However, 1a expressed in trans will suppress the production of a recombinant green fluorescent protein expressed from the nontranslated portions of BMV RNA1 and RNA2, suggesting that 1a may regulate translation from BMV RNAs. BMV replicase proteins 1a did not affect the accumulation of the BMV RNAs in the absence of RNA replication, unlike the situation reported for S. cerevisiae. This work demonstrates that the Agrobacterium-mediated gene delivery system can be used to study the cis- and trans-acting requirements for BMV RNA replication in plants and that significant differences can exist for BMV RNA replication in different hosts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.