ArticleNucleic acids research2005
Partial 13C isotopic enrichment of nucleoside monophosphates: useful reporters for NMR structural studies.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 9 citations in OpenAlex.
- The translational repressor Glorund uses interchangeable RNA recognition domains to recognize Drosophila nanos.Nucleic acids research · 2023Article
- Structure of RNA Stem Loop B from the Picornavirus Replication Platform.Biochemistry · 2017Article
- Role of helical constraints of the EBS1-IBS1 duplex of a group II intron on demarcation of the 5' splice site.RNA (New York, N.Y.) · 2014Article
- Biomass production of site selective 13C/15N nucleotides using wild type and a transketolase E. coli mutant for labeling RNA for high resolution NMR.Journal of biomolecular NMR · 2012Article
- Triple resonance experiments for aligned sample solid-state NMR of (13)C and (15)N labeled proteins.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2007Article
- Alternate-site isotopic labeling of ribonucleotides for NMR studies of ribose conformational dynamics in RNA.Journal of biomolecular NMR · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Analysis of the 13C isotopic labeling patterns of nucleoside monophosphates (NMPs) extracted from Escherichia coli grown in a mixture of C-1 and C-2 glucose is presented. By comparing our results to previous observations on amino acids grown in similar media, we have been able to rationalize the labeling pattern based on the well-known biochemistry of nucleotide biosynthesis. Except for a few notable absences of label (C4 in purines and C3' in ribose) and one highly enriched site (C1' in ribose), most carbons are randomly enriched at a low level (an average of 13%). These sparsely labeled NMPs give less complex NMR spectra than their fully isotopically labeled analogs due to the elimination of most 13C-13C scalar couplings. The spectral simplicity is particularly advantageous when working in ordered systems, as illustrated with guanosine diphosphate (GDP) bound to ADP ribosylation factor 1 (ARF1) aligned in a liquid crystalline medium. In this system, the absence of scalar couplings and additional long-range dipolar couplings significantly enhances signal to noise and resolution.
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Registered trials
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