ArticleMolecular biology of the cell2006
Actin is required for endocytosis at the apical surface of Madin-Darby canine kidney cells where ARF6 and clathrin regulate the actin cytoskeleton.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Apical clathrin-coated pits control the location, timing, and scale of microvillar growth.Cell reports · 2025Article
- Dynamic interplay between cell membrane tension and clathrin-mediated endocytosis.Biology of the cell · 2021Review
- Microtubules regulate brush border formation.Journal of cellular physiology · 2018Article
- ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Eps15R and clathrin regulate EphB2-mediated cell repulsion.Traffic (Copenhagen, Denmark) · 2018Article
- Membrane Transport across Polarized Epithelia.Cold Spring Harbor perspectives in biology · 2017Review
- Differentiation-dependent rearrangements of actin filaments and microtubules hinder apical endocytosis in urothelial cells.Histochemistry and cell biology · 2017Article
- Clathrin polymerization exhibits high mechano-geometric sensitivity.Soft matter · 2017Article
- A Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication.Journal of visualized experiments : JoVE · 2016Article
- Toxoplasma gondii Infection Promotes Epithelial Barrier Dysfunction of Caco-2 Cells.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2016Article
- Flow stimulated endocytosis in the proximal tubule.Current opinion in nephrology and hypertension · 2015Review
- Endocytic proteins drive vesicle growth via instability in high membrane tension environment.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- PIP5KIβ selectively modulates apical endocytosis in polarized renal epithelial cells.PloS one · 2013Article
- Depolymerization of cortical actin inhibits UT-A1 urea transporter endocytosis but promotes forskolin-stimulated membrane trafficking.American journal of physiology. Cell physiology · 2012Article
- Clathrin phosphorylation is required for actin recruitment at sites of bacterial adhesion and internalization.The Journal of cell biology · 2011Article
- Arf6 regulates AP-1B-dependent sorting in polarized epithelial cells.The Journal of cell biology · 2011Article
- Actin dynamics counteract membrane tension during clathrin-mediated endocytosis.Nature cell biology · 2011Article
- Vezatin, an integral membrane protein of adherens junctions, is required for the sound resilience of cochlear hair cells.EMBO molecular medicine · 2009Article
- Clathrin-independent endocytosis: from nonexisting to an extreme degree of complexity.Histochemistry and cell biology · 2008Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In epithelial cell lines, apical but not basolateral clathrin-mediated endocytosis has been shown to be affected by actin-disrupting drugs. Using electron and fluorescence microscopy, as well as biochemical assays, we show that the amount of actin dedicated to endocytosis is limiting at the apical surface of epithelia. In part, this contributes to the low basal rate of clathrin-dependent endocytosis observed at this epithelial surface. ARF6 in its GTP-bound state triggers the recruitment of actin from the cell cortex to the clathrin-coated pit to enable dynamin-dependent endocytosis. In addition, we show that perturbation of the apical endocytic system by expression of a clathrin heavy-chain mutant results in the collapse of microvilli. This phenotype was completely reversed by the expression of an ARF6-GTP-locked mutant. These observations indicate that concomitant to actin recruitment, the apical clathrin endocytic system is deeply involved in the morphology of the apical plasma membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.