ArticleMolecular biology of the cell2006
The CNA1 histone of the ciliate Tetrahymena thermophila is essential for chromosome segregation in the germline micronucleus.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 59 citations in OpenAlex.
- The mechanistic and evolutionary diversity of programmed DNA elimination.Nature reviews. Genetics · 2026Review
- Article
- Transcriptome analysis of the binucleate ciliateMolecular biology of the cell · 2023Article
- Exploration of the Nuclear Proteomes in the CiliateMicroorganisms · 2023Article
- A practical reference for studying meiosis in the model ciliateMarine life science & technology · 2022Review
- Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.Frontiers in cell and developmental biology · 2022Review
- The histone chaperone Nrp1 is required for chromatin stability and nuclear division in Tetrahymena thermophila.Epigenetics & chromatin · 2021Article
- Disruption of a ∼23-24 nucleotide small RNA pathway elevates DNA damage responses inMolecular biology of the cell · 2021Article
- Spatial constraints on chromosomes are instrumental to meiotic pairing.Journal of cell science · 2020Article
- Exploring the Histone Acetylation Cycle in the Protozoan ModelFrontiers in cell and developmental biology · 2020Review
- A chromatin-associated protein required for inducing and limiting meiotic DNA double-strand break formation.Nucleic acids research · 2018Article
- Whats, hows and whys of programmed DNA elimination inOpen biology · 2017Review
- A germline-limited piggyBac transposase gene is required for precise excision in Tetrahymena genome rearrangement.Nucleic acids research · 2017Article
- Evolutionary dynamics of the kinetochore network in eukaryotes as revealed by comparative genomics.EMBO reports · 2017Article
- DRH1, a p68-related RNA helicase gene, is required for chromosome breakage in Tetrahymena.Biology open · 2016Article
- Structure of the germline genome ofeLife · 2016Article
- Cyc17, a meiosis-specific cyclin, is essential for anaphase initiation and chromosome segregation in Tetrahymena thermophila.Cell cycle (Georgetown, Tex.) · 2016Article
- DNA deletion as a mechanism for developmentally programmed centromere loss.Nucleic acids research · 2016Article
- Methylation of histone H3K23 blocks DNA damage in pericentric heterochromatin during meiosis.eLife · 2014Article
- Epigenetics of ciliates.Cold Spring Harbor perspectives in biology · 2013Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Ciliated protozoans present several features of chromosome segregation that are unique among eukaryotes, including their maintenance of two nuclei: a germline micronucleus, which undergoes conventional mitosis and meiosis, and a somatic macronucleus that divides by an amitotic process. To study ciliate chromosome segregation, we have identified the centromeric histone gene in the Tetrahymena thermophila genome (CNA1). CNA1p specifically localizes to peripheral centromeres in the micronucleus but is absent in the macronucleus during vegetative growth. During meiotic prophase of the micronucleus, when chromosomes are stretched to twice the length of the cell, CNA1p is found localized in punctate spots throughout the length of the chromosomes. As conjugation proceeds, CNA1p appears initially diffuse, but quickly reverts to discrete dots in those nuclei destined to become micronuclei, whereas it remains diffuse and is gradually lost in developing macronuclei. In progeny of germline CNA1 knockouts, we see no defects in macronuclear division or viability of the progeny cells immediately following the knockout. However, within a few divisions, progeny show abnormal mitotic segregation of their micronucleus, with most cells eventually losing their micronucleus entirely. This study reveals a strong dependence of the germline micronucleus on centromeric histones for proper chromosome segregation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.