ArticleMolecular biology of the cell2006
Type I collagen structure regulates cell morphology and EGF signaling in primary rat hepatocytes through cAMP-dependent protein kinase A.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 77 citations in OpenAlex.
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- Extracellular Matrices as Bioactive Materials for In Situ Tissue Regeneration.Pharmaceutics · 2023Review
- Effect of Phytic Acid Addition on the Structure of Collagen-Hyaluronic Acid Composite Gel.Gels (Basel, Switzerland) · 2023Article
- Significance of the p38MAPK-CRP2 axis in myofibroblastic phenotypic transition.Cell structure and function · 2023Article
- Correlations on the Structure and Properties of Collagen Hydrogels Produced by E-Beam Crosslinking.Materials (Basel, Switzerland) · 2022Article
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- Bioinspired liver scaffold design criteria.Organogenesis · 2018Article
- Extracellular matrix with defective collagen cross-linking affects the differentiation of bone cells.PloS one · 2018Article
- Decellularization and Solubilization of Porcine Liver for Use as a Substrate for Porcine Hepatocyte Culture: Method Optimization and Comparison.Cell transplantation · 2017Article
- Monocyte Adhesion, Migration, and Extracellular Matrix Breakdown Are Regulated by Integrin αVβ3 inJournal of immunology (Baltimore, Md. : 1950) · 2017Article
- Role of the prostaglandin E2 receptor agonists in TGF-β1-induced mesangial cell damage.Bioscience reports · 2016Article
- Physiological ranges of matrix rigidity modulate primary mouse hepatocyte function in part through hepatocyte nuclear factor 4 alpha.Hepatology (Baltimore, Md.) · 2016Article
- Differentiation of Murine Bone Marrow-Derived Smooth Muscle Progenitor Cells Is Regulated by PDGF-BB and Collagen.PloS one · 2016Article
- Atomic force microscopy investigation of the interaction of low-level laser irradiation of collagen thin films in correlation with fibroblast response.Lasers in medical science · 2015Article
- Photopatterning of hydrogel scaffolds coupled to filter materials using stereolithography for perfused 3D culture of hepatocytes.Biotechnology and bioengineering · 2015Article
- Substrate stiffness regulates primary hepatocyte functions.RSC advances · 2015Article
- Novel use for polyvinylpyrrolidone as a macromolecular crowder for enhanced extracellular matrix deposition and cell proliferation.Tissue engineering. Part C, Methods · 2014Article
- Characterizing the effects of heparin gel stiffness on function of primary hepatocytes.Tissue engineering. Part A · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adhesion to type 1 collagen elicits different responses dependent on whether the collagen is in fibrillar (gel) or monomeric form (film). Hepatocytes adherent to collagen film spread and proliferate, whereas those adherent to collagen gel remain rounded and growth arrested. To explore the role of potential intracellular inhibitory signals responsible for collagen gel-mediated growth arrest, cAMP-dependent protein kinase A (PKA) was examined in hepatocytes adherent to collagen film or gel. PKA activity was higher in hepatocytes on collagen gel than on film during G1 of the hepatocyte cell cycle. Inhibition of PKA using H89 increased cell spreading on collagen gel in an EGF-dependent manner, whereas activation of PKA using 8-Br-cAMP decreased cell spreading on collagen film. PKA inhibition also restored ERK activation, cyclin D1 expression and G1-S progression on collagen gel, but had no effect on cells adherent to collagen film. Analysis of EGF receptor phosphorylation revealed that adhesion to collagen gel alters tyrosine phosphorylation of the EGF receptor, leading to reduced phosphorylation of tyrosine residue 845, which was increased by inhibition of PKA. These results demonstrate that fibrillar type 1 collagen can actively disrupt cell cycle progression by inhibiting specific signals from the EGF receptor through a PKA-dependent pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.