ArticleAntimicrobial agents and chemotherapy2005
Fitness cost due to mutations in the 16S rRNA associated with spectinomycin resistance in Chlamydia psittaci 6BC.
Article in Antimicrobial agents and chemotherapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 47 citations in OpenAlex.
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- An overview of genes and mutations associated with Chlamydiae species' resistance to antibiotics.Annals of clinical microbiology and antimicrobials · 2021Review
- The rise and the fall of aMicrobial genomics · 2021Article
- Mini Review: Antimicrobial Control of Chlamydial Infections in Animals: Current Practices and Issues.Frontiers in microbiology · 2019Article
- Beyond Tryptophan Synthase: Identification of Genes That Contribute to Chlamydia trachomatis Survival during Gamma Interferon-Induced Persistence and Reactivation.Infection and immunity · 2016Article
- Review
- The genetic basis of the fitness costs of antimicrobial resistance: a meta-analysis approach.Evolutionary applications · 2015Article
- The fitness costs of antibiotic resistance mutations.Evolutionary applications · 2015Review
- Article
- The role of the mitochondrial ribosome in human disease: searching for mutations in 12S mitochondrial rRNA with high disruptive potential.Human molecular genetics · 2014Article
- Fitness factors in vibrios: a mini-review.Microbial ecology · 2013Review
- A novel co-infection model with Toxoplasma and Chlamydia trachomatis highlights the importance of host cell manipulation for nutrient scavenging.Cellular microbiology · 2013Article
- Neisseria gonorrhoeae strain with high-level resistance to spectinomycin due to a novel resistance mechanism (mutated ribosomal protein S5) verified in Norway.Antimicrobial agents and chemotherapy · 2013Article
- Mutation in ribosomal protein S5 leads to spectinomycin resistance in Neisseria gonorrhoeae.Frontiers in microbiology · 2013Article
- Resistance to a novel antichlamydial compound is mediated through mutations in Chlamydia trachomatis secY.Antimicrobial agents and chemotherapy · 2012Article
- Whole-genome analysis of diverse Chlamydia trachomatis strains identifies phylogenetic relationships masked by current clinical typing.Nature genetics · 2012Article
- Spontaneous spectinomycin resistance mutations detected after biolistic transformation of Daucus carota L.Physiology and molecular biology of plants : an international journal of functional plant biology · 2011Article
- Infectivity acts as in vivo selection for maintenance of the chlamydial cryptic plasmid.Infection and immunity · 2011Article
- No apparent costs for facultative antibiotic production by the soil bacterium Pseudomonas fluorescens Pf0-1.PloS one · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The fitness cost of a resistance determinant is the primary parameter that determines its frequency in vivo. As a model for analysis of the impact of drug resistance mutations on the intracellular life cycle of Chlamydia spp., we studied the growth of four genetically defined spectinomycin-resistant (Spc(r)) clonal variants of Chlamydia psittaci 6BC isolated in the plaque assay. The development of each variant was monitored over 46 h postinfection in the absence of drug, either in pure culture or in 1:1 competition with the parent strain. Spc(r) mutations in the 16S rRNA gene at positions 1191 and 1193 were associated with a marked impairment of C.psittaci biological fitness, and the bacteria were severely out-competed by the wild-type parent. In contrast, mutations at position 1192 had minor effects on the bacterial life cycle, allowing the resistant isolates to compete more efficiently with the wild-type strain. Thus, mutations with a wide range of fitness costs can be selected in the plaque assay, providing a new strategy for prediction and monitoring of the emergence of antibiotic resistance in chlamydiae. So far, drug resistance has not been a serious threat for the treatment of chlamydial infections. Tetracycline is an effective antichlamydial drug that targets 16S rRNA. Attempts to isolate spontaneous tetracycline-resistant mutants of C. psittaci 6BC revealed a frequency <3 x 10(-9). We suggest that the rarity of genotypic antibiotic resistance among chlamydial clinical isolates reflects the deleterious effects of such mutations on the fitness of these obligate intracellular bacteria in the host.
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