ArticleMarine biotechnology (New York, N.Y.)
Production and characterization of a continuous embryonic cell line from sea bass (Dicentrarchus labrax L.).
Article in Marine biotechnology (New York, N.Y.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- Towards Cost-Effective and Sustainable Media Formulations for Terrestrial and Aquatic Cellular Agriculture.Foods (Basel, Switzerland) · 2026Review
- Antarctic Fish Antimicrobial Peptides Active Against Bacterial and Viral Pathogens of Aquacultural Importance.Antibiotics (Basel, Switzerland) · 2026Article
- Comparative Analysis of CRISPR/Cas9 Delivery Methods in Marine Teleost Cell Lines.International journal of molecular sciences · 2025Article
- Mimicking the myoseptum in cultivated fish by manufacturing edible microalgae-rich nanofibers.NPJ science of food · 2025Article
- Low-cost food-grade alternatives for serum albumins in FBS-free cell culture media.Scientific reports · 2025Article
- Microalgae-enriched (bio)inks for 3D bioprinting of cultured seafood.NPJ science of food · 2025Article
- Artificial intelligence and machine learning applications for cultured meat.Frontiers in artificial intelligence · 2024Review
- Repurposing biomedical muscle tissue engineering for cellular agriculture: challenges and opportunities.Trends in biotechnology · 2023Review
- Differentiation and Maturation of Muscle and Fat Cells in Cultivated Seafood: Lessons from Developmental Biology.Marine biotechnology (New York, N.Y.) · 2023Review
- Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives.Biomolecules · 2022Review
- Establishment and characterization of a cell line from the Chinese soft-shelled turtle Pelodiscus sinensis.In vitro cellular & developmental biology. Animal · 2016Article
- Anti-genotoxic ability of α-tocopherol and Anthocyanin to counteract fish DNA damage induced by musk xylene.Ecotoxicology (London, England) · 2015Article
- RAPD-PCR analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from Dicentrarchus labrax.Cytotechnology · 2014Article
- Characteristics of established KSG cells derived from the scorpionfish Sebastiscus marmoratus: what happens under the hydrostatic pressure like the deep sea?In vitro cellular & developmental biology. Animal · 2014Article
- Establishment, characterization, and virus susceptibility of a new marine cell line from red spotted grouper (Epinephelus akaara).Marine biotechnology (New York, N.Y.)Article
- Development and characterization of two new cell lines from milkfish (Chanos chanos) and grouper (Epinephelus coioides) for virus isolation.Marine biotechnology (New York, N.Y.)Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Continuous cell lines represent an important tool both for biological studies and for their applications in marine biotechnology. In this article we describe the production and characterization of a continuous adherent cell line, named DLEC, derived from early embryos of the European sea bass Dicentrarchus labrax L. (Actinopterygii, Moronidae). Cells were obtained by disrupting 2- to 12-hour-old embryos and culturing resulting cells at 18 degrees C in RPMI medium containing 5% fetal calf serum (FCS) and 10% supernatant fraction of the embryo homogenate. After 8 weeks culture medium was replaced with Liebovitz's L15 medium containing 10% FCS and DLEC cells started proliferation. Subsequently, they were continuously cultured until the 50th passage without evident changes in their morphology. DLEC cells show a fibroblast-like shape and a modal chromosome number of 48, as do the wild-type cells; conversely the constant presence of six to nine meta-submetacentric elements in the karyotype (vs. zero to two in the wild-type) indicates the occurrence of chromosomal rearrangements during stabilization. DLEC cells are sensitive to substances known to induce differentiation of mammalian cells such as retinoic acid and phorbol esters. They have been transfected using liposomes with a commercial plasmid vector containing a reporter gene, thus suggesting a possible importance as an alternative expression system of recombinant vertebrate proteins in teleost cells.
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