ArticlePlant cell reports2006
Low light and low ammonium are key factors for guayule leaf tissue shoot organogenesis and transformation.
Article in Plant cell reports, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- How does light regulate plant regeneration?Frontiers in plant science · 2024Review
- Downregulation of Squalene Synthase Broadly Impacts Isoprenoid Biosynthesis in Guayule.Metabolites · 2022Article
- Early Low-Fluence Red Light or Darkness Modulates the Shoot Regeneration Capacity of ExcisedPlants (Basel, Switzerland) · 2020Article
- Downregulation of a CYP74 Rubber Particle Protein Increases Natural Rubber Production inFrontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new method has been developed for guayule tissue culture and transformation. Guayule leaf explants have a poor survival rate when placed on normal MS medium and under normal culture room light conditions. Low light and low ammonium treatment greatly improved shoot organogenesis and transformation from leaf tissues. Using this method, a 35S promoter driven BAR gene and an ubiquitin-3 promoter driven GUS gene (with intron) have been successfully introduced into guayule. These transgenic guayule plants were resistant to the herbicide ammonium-glufosinate and were positive to GUS staining. Molecular analysis showed the expected band and signal in all GUS positive transformants. The transformation efficiency with glufosinate selection ranged from 3 to 6%. Transformation with a pBIN19-based plasmid containing a NPTII gene and then selection with kanamycin also works well using this method. The ratio of kanamycin-resistant calli to total starting explants reached 50% in some experiments.
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Registered trials
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