ArticlePlant molecular biology2005
Functional characterization of a melon alcohol acyl-transferase gene family involved in the biosynthesis of ester volatiles. Identification of the crucial role of a threonine residue for enzyme activity*.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 199 citations in OpenAlex.
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- A MYB-WRKY feedback module activates MdAAT2‑like to regulate aromatic ester biosynthesis during apple ripening.Molecular horticulture · 2026Article
- Plant Genetic Engineering: Technological Pathways, Application Scenarios, and Future Directions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Changes in Alcohol dehydrogenase gene expression throughout strawberry fruit ripening process.Plant molecular biology · 2026Article
- Salicylic acid biosynthesis via the PAL pathway requires benzaldehyde synthase and a benzyl salicylate-specific esterase.The Plant cell · 2025Article
- Mechanism mediating the biosynthesis of the anti-insect volatile (Z)-3-hexenyl acetate in Acacia confusa Merr., an intercropping plant in tea plantations.Molecular horticulture · 2025Article
- Phenolic metabolism in Sarcandra glabra is mediated by distinct BAHD hydroxycinnamoyltransferases.The Plant journal : for cell and molecular biology · 2025Article
- Untargeted flower volatilome profiling highlights differential pollinator attraction strategies in muscadine.Frontiers in plant science · 2025Article
- Comprehensive aroma profiles and the underlying molecular mechanisms in six grape varieties with different flavors.Frontiers in plant science · 2025Article
- Dynamic Changes of Active Components and Volatile Organic Compounds inFoods (Basel, Switzerland) · 2024Article
- Genome-Wide Identification and Expression Pattern Analysis of BAHD Acyltransferase Family inInternational journal of molecular sciences · 2024Article
- Chemical and genetic basis of orange flavor.Science advances · 2024Article
- Genetic regulation of volatile production in two melon introgression line collections with contrasting ripening behavior.Horticulture research · 2024Article
- Article
- Genome-wide identification and expression analysis of the BAHD gene family inFrontiers in genetics · 2024Article
- Expression of alcohol acyltransferase is a potential determinant of fruit volatile ester variations in Capsicum.Plant cell reports · 2023Article
- Alcohol acyltransferases for the biosynthesis of esters.Biotechnology for biofuels and bioproducts · 2023Review
- Changes in the VOC of Fruits at Different Refrigeration Stages of 'Ruixue' and the Participation of CarboxylesteraseFoods (Basel, Switzerland) · 2023Article
- Thorough Characterization ofFoods (Basel, Switzerland) · 2023Article
- Genome-Wide Identification ofLife (Basel, Switzerland) · 2022Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Volatile esters, a major class of compounds contributing to the aroma of many fruit, are synthesized by alcohol acyl-transferases (AAT). We demonstrate here that, in Charentais melon (Cucumis melo var. cantalupensis), AAT are encoded by a gene family of at least four members with amino acid identity ranging from 84% (Cm-AAT1/Cm-AAT2) and 58% (Cm-AAT1/Cm-AAT3) to only 22% (Cm-AAT1/Cm-AAT4). All encoded proteins, except Cm-AAT2, were enzymatically active upon expression in yeast and show differential substrate preferences. Cm-AAT1 protein produces a wide range of short and long-chain acyl esters but has strong preference for the formation of E-2-hexenyl acetate and hexyl hexanoate. Cm-AAT3 also accepts a wide range of substrates but with very strong preference for producing benzyl acetate. Cm-AAT4 is almost exclusively devoted to the formation of acetates, with strong preference for cinnamoyl acetate. Site directed mutagenesis demonstrated that the failure of Cm-AAT2 to produce volatile esters is related to the presence of a 268-alanine residue instead of threonine as in all active AAT proteins. Mutating 268-A into 268-T of Cm-AAT2 restored enzyme activity, while mutating 268-T into 268-A abolished activity of Cm-AAT1. Activities of all three proteins measured with the prefered substrates sharply increase during fruit ripening. The expression of all Cm-AAT genes is up-regulated during ripening and inhibited in antisense ACC oxidase melons and in fruit treated with the ethylene antagonist 1-methylcyclopropene (1-MCP), indicating a positive regulation by ethylene. The data presented in this work suggest that the multiplicity of AAT genes accounts for the great diversity of esters formed in melon.
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