ArticleProceedings of the National Academy of Sciences of the United States of America2005
3D structure of eukaryotic flagella in a quiescent state revealed by cryo-electron tomography.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
74 citing papers in PubMed.
- Advances in high-resolution imaging of centrioles and cilia.Journal of cell science · 2025Review
- A generalizable and targeted molecular biopsy approach for in situ cryogenic electron tomography of vitreous brain tissue.Cell reports methods · 2025Article
- Structural determination and modeling of ciliary microtubules.Acta crystallographica. Section D, Structural biology · 2024Review
- In-cell structural insight into the stability of sperm microtubule doublet.Cell discovery · 2023Article
- Innovative Imaging Techniques: A Conceptual Exploration of Multi-Modal Raman Light Sheet Microscopy.Micromachines · 2023Article
- NovelThe world journal of men's health · 2022Review
- Subtomogram analysis: The sum of a tomogram's particles reveals molecular structureJournal of structural biology: X · 2022Article
- Structural organization of the C1b projection within the ciliary central apparatus.Journal of cell science · 2021Article
- Structural insights into the cause of humanMolecular biology of the cell · 2021Article
- Article
- Force-Generating Mechanism of Axonemal Dynein in Solo and Ensemble.International journal of molecular sciences · 2020Review
- Post-ejaculatory modifications to sperm (PEMS).Biological reviews of the Cambridge Philosophical Society · 2020Review
- Investigating eukaryotic cells with cryo-ET.Molecular biology of the cell · 2020Article
- Electron cryotomography of intact motile cilia defines the basal body to axoneme transition.The Journal of cell biology · 2020Article
- Article
- Article
- Review
- It takes two (centrioles) to tango.Reproduction (Cambridge, England) · 2019Review
- Electron tomography-a tool for ultrastructural 3D visualization in cell biology and histology.Wiener medizinische Wochenschrift (1946) · 2018Review
- Hybrid Structured Illumination Expansion Microscopy Reveals Microbial Cytoskeleton Organization.ACS nano · 2017Article
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
We have used cryo-electron tomography to investigate the 3D structure and macromolecular organization of intact, frozen-hydrated sea urchin sperm flagella in a quiescent state. The tomographic reconstructions provide information at a resolution better than 6 nm about the in situ arrangements of macromolecules that are key for flagellar motility. We have visualized the heptameric rings of the motor domains in the outer dynein arm complex and determined that they lie parallel to the plane that contains the axes of neighboring flagellar microtubules. Both the material associated with the central pair of microtubules and the radial spokes display a plane of symmetry that helps to explain the planar beat pattern of these flagella. Cryo-electron tomography has proven to be a powerful technique for helping us understand the relationships between flagellar structure and function and the design of macromolecular machines in situ.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.