ArticleNucleic acids research2005
Rearranging the centromere of the human Y chromosome with phiC31 integrase.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Site-specific DNA insertion into the human genome with engineered recombinases.Nature biotechnology · 2025Article
- There and turn back again: the application of phage serine integrases in eukaryotic systems.Frontiers in bioengineering and biotechnology · 2025Review
- Article
- Comparison and optimization of ten phage encoded serine integrases for genome engineering in Saccharomyces cerevisiae.BMC biotechnology · 2016Article
- Construction of a stepwise gene integration system by transient expression of actinophage R4 integrase in cyanobacterium Synechocystis sp. PCC 6803.Molecular genetics and genomics : MGG · 2014Article
- New applications for phage integrases.Journal of molecular biology · 2014Review
- Expanding the scope of site-specific recombinases for genetic and metabolic engineering.Biotechnology and bioengineering · 2014Review
- Accuracy and efficiency define Bxb1 integrase as the best of fifteen candidate serine recombinases for the integration of DNA into the human genome.BMC biotechnology · 2013Article
- A versatile in vivo system for directed dissection of gene expression patterns.Nature methods · 2011Article
- In vivo evaluation of PhiC31 recombinase activity using a self-excision cassette.Nucleic acids research · 2008Article
- Highly efficient in vitro site-specific recombination system based on streptomyces phage phiBT1 integrase.Journal of bacteriology · 2008Article
- A motif in the C-terminal domain of phiC31 integrase controls the directionality of recombination.Nucleic acids research · 2008Article
- Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage phiBT1.Nucleic acids research · 2008Article
- Active integration: new strategies for transgenesis.Transgenic research · 2007Article
- Sequences in attB that affect the ability of phiC31 integrase to synapse and to activate DNA cleavage.Nucleic acids research · 2007Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We have investigated the ability of the integrase from the Streptomyces phiC31 'phage to either delete or invert 1 Mb of DNA around the centromere of the human Y chromosome in chicken DT40 hybrid somatic cells. Reciprocal and conservative site-specific recombination was observed in 54% of cells expressing the integrase. The sites failed to recombine in the remaining cells because the sites had been damaged. The sequences of the damaged sites indicated that the damage arose as a result of repair of recombination intermediates by host cell pathways. The liability of recombination intermediates to damage is consistent with what is known about the mechanism of serine recombinase reactions. The structures of the products of the chromosome rearrangements were consistent with the published sequence of the Y chromosome indicating that the assembly of the highly repeated region between the sites is accurate to a resolution of about 50 kb. Mini-chromosomes lacking a centromere were not recovered which also suggested that neo-centromere formation occurs infrequently in vertebrate somatic cells. No ectopic recombination was observed between a phiC31 integrase attB site and the chicken genome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.