ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2006
An in vitro model for characterizing the post-migratory cranial neural crest cells of the first branchial arch.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Augmented BMP signaling commits cranial neural crest cells to a chondrogenic fate by suppressing autophagic β-catenin degradation.Science signaling · 2021Article
- Mesenchymal stem cell-based tissue regeneration therapies for periodontitis.Regenerative therapy · 2020Review
- Review
- Activation ofInternational journal of molecular sciences · 2018Article
- mTOR acts as a pivotal signaling hub for neural crest cells during craniofacial development.PLoS genetics · 2018Article
- Influence of prenatal EGCG treatment and Dyrk1a dosage reduction on craniofacial features associated with Down syndrome.Human molecular genetics · 2016Article
- Comparison of P75 NTR-positive and -negative etcomesenchymal stem cell odontogenic differentiation through epithelial-mesenchymal interaction.Cell proliferation · 2016Article
- Applications of Mesenchymal Stem Cells and Neural Crest Cells in Craniofacial Skeletal Research.Stem cells international · 2016Review
- Stem Cells in Teeth and Craniofacial Bones.Journal of dental research · 2015Review
- FGF8 signaling sustains progenitor status and multipotency of cranial neural crest-derived mesenchymal cells in vivo and in vitro.Journal of molecular cell biology · 2015Article
- Neural crest stem cells: discovery, properties and potential for therapy.Cell research · 2012Review
- V-ATPase-dependent ectodermal voltage and pH regionalization are required for craniofacial morphogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2011Article
- Induction of the Nrf2-driven antioxidant response by tert-butylhydroquinone prevents ethanol-induced apoptosis in cranial neural crest cells.Biochemical pharmacology · 2010Article
- Stem cell property of postmigratory cranial neural crest cells and their utility in alveolar bone regeneration and tooth development.Stem cells (Dayton, Ohio) · 2009Article
- TGF-beta type I receptor Alk5 regulates tooth initiation and mandible patterning in a type II receptor-independent manner.Developmental biology · 2008Article
- A nonneural epithelial domain of embryonic cranial neural folds gives rise to ectomesenchyme.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- A neural crest deficit in Down syndrome mice is associated with deficient mitotic response to Sonic hedgehog.Mechanisms of developmentArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The cranial neural crest (CNC) is a transient cell population that originates at the crest of the neural fold and gives rise to multiple cell types during craniofacial development. Traditionally, researchers have used tissue explants, such as the neural tube, to obtain primary neural crest cells for their studies. However, this approach has inevitably resulted in simultaneous isolation of neural and non-neural crest cells as both of these cells migrate away from tissue explants. Using the Wnt1-Cre/R26R mouse model, we have obtained a pure population of neural crest cells and established a primary CNC cell culture system in which the cell culture medium best supports the proliferation of E10.5 first branchial arch CNC cells and maintains these cells in their undifferentiated state. Differentiation of CNC cells can be initiated by switching to a differentiation medium. In this model, cultured CNC cells can give rise to neurons, glial cells, osteoblasts, and other cell types, faithfully mimicking the differentiation process of the post-migratory CNC cells in vivo. Taken together, our study shows that the Wnt1-Cre/R26R mouse first branchial arch provides an excellent model for obtaining post-migratory neural crest cells free of any mesodermal contaminants. The cultured neural crest cells are under sustained proliferative, undifferentiated, or lineage-enhanced conditions, hence, serving as a tool for the investigation of the regulatory mechanism of CNC cell fate determination in normal and abnormal craniofacial development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.