ArticleTransgenic research2005
Agroinfiltration as a tool for transient expression of cre recombinase in vivo.
Article in Transgenic research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- GmMDE genes bridge the maturity gene E1 and florigens in photoperiodic regulation of flowering in soybean.Plant physiology · 2022Article
- Long-Term Potato Virus X (PVX)-Based Transient Expression of Recombinant GFP Protein inPlants (Basel, Switzerland) · 2021Article
- An improved and efficient method of Agrobacterium syringe infiltration for transient transformation and its application in the elucidation of gene function in poplar.BMC plant biology · 2021Article
- An Efficient Gene Excision System in Maize.Frontiers in plant science · 2020Article
- The pollen- and embryo-specific Arabidopsis DLL promoter bears good potential for application in marker-free Cre/loxP self-excision strategy.Plant cell reports · 2015Article
- Article
- Less is more: strategies to remove marker genes from transgenic plants.BMC biotechnology · 2013Review
- Agroinfiltration of intact leaves as a method for the transient and stable transformation of saponin producing Maesa lanceolata.Plant cell reports · 2012Article
- Recombinase technology: applications and possibilities.Plant cell reports · 2011Review
- Multiple regulatory elements in the Arabidopsis NIA1 promoter act synergistically to form a nitrate enhancer.Plant physiology · 2010Article
- Cre-mediated seed-specific transgene excision in tobacco.Plant molecular biology · 2010Article
- PhiC31 recombination system demonstrates heritable germinal transmission of site-specific excision from the Arabidopsis genome.BMC biotechnology · 2010Article
- Developmentally regulated site-specific marker gene excision in transgenic B. napus plants.Plant cell reports · 2009Article
- ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome.Transgenic research · 2009Article
- Marker-free transgenic plants through genetically programmed auto-excision.Plant physiology · 2007Article
- Direct creation of marker-free tobacco plants from agroinfiltrated leaf discs.Plant cell reports · 2007Article
- A Cre/loxP-mediated self-activating gene excision system to produce marker gene free transgenic soybean plants.Plant molecular biology · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agroinfiltration was used to express transiently cre recombinase from bacteriophage P1 in planta. Activation of gfp expression after cre-mediated excision of a bar intervening sequence served as a marker to monitor site-specific recombination events in lox-target N. benthamiana plants. Gfp expressing regenerants from A. tumefaciens infiltrated leaves were obtained with an efficiency of about 34%. In 20% of the regenerants bar gene excision was due to the expression of stably integrated cre gene, whereas in 14% of plants site-specific recombination was a consequence of transient cre expression. Phenotypic and molecular data indicated that the recombined state has been transferred to the T(1 )generation. These results demonstrate the suitability of agroinfiltration for the expression of cre recombinase in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.