ArticleTransgenic research2005
Production of human serum albumin by sugar starvation induced promoter and rice cell culture.
Article in Transgenic research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 86 citations in OpenAlex.
- A single donor cassette enables site-specific knock-in at either the αAmy3 or αAmy8 locus in rice cells via CRISPR/Cas9.Applied microbiology and biotechnology · 2025Article
- Expression of an antimicrobial peptide persulcatusin fused with calmodulin in rice cultured cells.Transgenic research · 2025Article
- High-yield BMP2 expression in rice cells via CRISPR and endogenous αAmy3 promoter.Applied microbiology and biotechnology · 2024Article
- Production of Mature Recombinant Human Activin A in Transgenic Rice Cell Suspension Culture.Current issues in molecular biology · 2024Article
- Purslane (Portulaca oleracea L.) as a novel green-bioreactor for expression of human serum albumin (HSA) gene.Transgenic research · 2022Article
- Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells.Plant methods · 2021Article
- Expression of Recombinant Human Octamer-Binding Transcription Factor 4 in Rice Suspension Cells.International journal of molecular sciences · 2021Article
- Development of Systems for the Production of Plant-Derived Biopharmaceuticals.Plants (Basel, Switzerland) · 2019Review
- Putting the Spotlight Back on Plant Suspension Cultures.Frontiers in plant science · 2016Review
- Efficient Secretion of Recombinant Proteins from Rice Suspension-Cultured Cells Modulated by the Choice of Signal Peptide.PloS one · 2015Article
- The modified rice αAmy8 promoter confers high-level foreign gene expression in a novel hypoxia-inducible expression system in transgenic rice seedlings.Plant molecular biology · 2014Article
- Immunotoxicity assessment of rice-derived recombinant human serum albumin using human peripheral blood mononuclear cells.PloS one · 2014Article
- Expression and purification of recombinant human serum albumin from selectively terminable transgenic rice.Journal of Zhejiang University. Science. B · 2013Article
- Improving pharmaceutical protein production in Oryza sativa.International journal of molecular sciences · 2013Review
- Large-scale production of functional human serum albumin from transgenic rice seeds.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Design of gene constructs for transgenic maize.Methods in molecular biology (Clifton, N.J.) · 2009Review
- Tumor targeting of humanized fragment antibody secreted from transgenic rice cell suspension culture.Plant molecular biology · 2008Article
- Plants as bioreactors: Recent developments and emerging opportunities.Biotechnology advancesReview
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human serum albumin (HSA) is the most widely used clinical serum protein. Currently, commercial HSA can only be obtained from human plasma, due to lack of commercially feasible recombinant protein expression systems. In this study, inducible expression and secretion of HSA by transformed rice suspension cell culture was established. Mature form of HSA was expressed under the control of the sucrose starvation-inducible rice alpha Amy3 promoter, and secretion of HSA into the culture medium was achieved by using the alpha Amy3 signal sequence. High concentrations of HSA were secreted into culture medium in a short time (2-4 days) by sucrose depletion after cell concentrations had reached a peak density in culture medium containing sucrose. The recombinant HSA had the same electrophoretic mobility as commercial HSA and was stable and free from apparent proteolysis in the culture medium. In a flask scale culture with repeated sucrose provision-depletion cycles, HSA was stably produced with yields up to 11.5% of total medium proteins or 15 mg/L per cycle after each sucrose provision-depletion cycle. A bubble column type bioreactor was designed for production of HSA. In the bioreactor scale culture, HSA was produced with yields up to 76.4 mg/L 4 days after sucrose depletion. HSA was purified from the culture medium to high purity by a simple purification scheme. Enrichment of HSA in culture medium simplifies downstream purification, minimizes protease degradation, and may reduce production cost. The combination of a DNA construct containing the alpha Amy3 promoter and signal sequence, and the use of a rice suspension cell culture can provide an effective system for the production of recombinant pharmaceutical proteins.
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Registered trials
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