ArticleThe Journal of membrane biology2005
Fast-activating channel controls cation fluxes across the native chloroplast envelope.
Article in The Journal of membrane biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 28 citations in OpenAlex.
- PLASTID ENVELOPE ION CHANNELS (PEC1/2) link CaProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Cellular aspects of NaQuantitative plant biology · 2026Review
- Two plastid POLLUX ion channel-like proteins are required for stress-triggered stromal Ca2+release.Plant physiology · 2021Article
- Proton Gradients and Proton-Dependent Transport Processes in the Chloroplast.Frontiers in plant science · 2016Review
- Organellar channels and transporters.Cell calcium · 2015Review
- Ion Channels in Native Chloroplast Membranes: Challenges and Potential for Direct Patch-Clamp Studies.Frontiers in physiology · 2015Review
- Plastidial transporters KEA1, -2, and -3 are essential for chloroplast osmoregulation, integrity, and pH regulation in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Function and evolution of channels and transporters in photosynthetic membranes.Cellular and molecular life sciences : CMLS · 2014Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A prerequisite for photosynthetic CO(2) fixation is the maintenance of alkaline pH in the stroma. This is achieved by H(+) pumping from the stroma to the cytosol, electrically balanced by an influx of cations through some unidentified non-selective envelope channels. In this study, the patch-clamp technique was applied to isolated Pisum sativum L. (pea) chloroplasts, and a fast-activating chloroplast cation (FACC) channel was discovered in the native envelope. This channel opens within a few milliseconds upon voltage steps to large positive or negative potentials. Remarkably, the single-channel conductance increased fivefold, from approximately 40 pS to approximately 200 pS (symmetric 250 mM KCl), upon a potential change from zero to +/- 200 mV. The FACC channel conducts all physiologically essential inorganic cations (K(+), Na(+), Ca(2+), Mg(2+)) with little preference. An increase of stromal pH from 7.3 to 8.0, mimicking dark-light transition, caused about a 2-fold decrease of the FACC channel activity within a physiologically relevant potential range. The FACC channel was completely and irreversibly blocked by Gd(3+). Based on the estimated transport capacity of the whole chloroplast population of FACC channels together with the envelope H(+)-ATPases, these channels can mediate electroneutral K(+)/H(+) exchange across the envelope, enabling stroma alkalinization, thereby allowing an optimal photosynthetic performance.
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