ArticlePlant molecular biology2005
Analysis of RNA silencing in agroinfiltrated leaves of Nicotiana benthamiana and Nicotiana tabacum.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 90 citations in OpenAlex.
- A synthetic expression system for orthogonal gene expression in Nicotiana benthamiana.Plant molecular biology · 2026Article
- Strain, procedures, and tools for reproducible genetic transformation and genome editing of the emerging plant model Spirodela polyrhiza.The New phytologist · 2026Article
- Recovery of RNA-dependent RNA polymerase 6 gene-knockout phenotypes in Nicotiana benthamiana via in vivo generation of inverted repeat construct of the trans-acting short interference RNA3 sequence.The Plant journal : for cell and molecular biology · 2025Article
- RNA interference: a promising biotechnological approach to combat plant pathogens, mechanism and future prospects.World journal of microbiology & biotechnology · 2024Review
- Evaluation of the RNA Silencing Suppression Activity of Three Cherry Virus F-Encoded Proteins.Plants (Basel, Switzerland) · 2024Article
- CRISPR/Cas9-mediated knockout of the DCL2 and DCL4 genes in Nicotiana benthamiana and its productivity of recombinant proteins.Plant cell reports · 2022Article
- Agroinfiltration Mediated Scalable Transient Gene Expression in Genome Edited Crop Plants.International journal of molecular sciences · 2021Review
- Application of virus-induced gene silencing in Andrographis paniculata, an economically important medicinal plant.Protoplasma · 2021Article
- An Arabidopsis downy mildew non-RxLR effector suppresses induced plant cell death to promote biotroph infection.Journal of experimental botany · 2021Article
- Systemic GFP silencing is associated with high transgene expression in Nicotiana benthamiana.PloS one · 2021Article
- The Whys and Wherefores of Transitivity in Plants.Frontiers in plant science · 2020Review
- Molecular basis of transitivity in plant RNA silencing.Molecular biology reports · 2019Review
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- Induction of Transgene Suppression in Plants via External Application of Synthetic dsRNA.International journal of molecular sciences · 2019Article
- DCL-suppressed Nicotiana benthamiana plants: valuable tools in research and biotechnology.Molecular plant pathology · 2019Article
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- RNAi-mediated endogene silencing in strawberry fruit: detection of primary and secondary siRNAs by deep sequencing.Plant biotechnology journal · 2017Article
- Two homologous host proteins interact with potato virus X RNAs and CPs and affect viral replication and movement.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study we analyse several aspects of cytoplasmic RNA silencing by agroinfiltration of DNA constructs encoding single- and double-stranded RNAs derived from a GFP transgene and from the endogenous Virp1 gene. Both types of inductors resulted after 2-4 days in much higher concentration of siRNAs in the agroinfiltrated zone than normally seen during systemic silencing. More specifically, infiltration of two transgene hairpin constructs resulted in elevated levels of siRNAs. However, differences between the two constructs were observed: the antisense-sense arrangement was more effective than the sense-antisense order. For both double-stranded forms, we observed a relative increase of the 24-mer size class of siRNAs. When a comparable hairpin construct of the endogenous Virp1 gene was assayed, the portion of the 24-mer siRNA class remained low as observed for all kinds of single-stranded inducers. The lack of increase of Virp1-derived 24-mers was independent of the expression level, as demonstrated by agroinfiltration into a transgenic plant that overexpressed Virp1 and showed the same pattern. Using transducer constructs, we could detect within a week transitive silencing from GFP to GUS sequences in the infiltrated zone and in either direction 5'-3' and 3'-5'. Conversely, for the endogenous Virp1 gene neither transitive silencing nor the induction of systemic silencing could be observed. These results are discussed in view of the current models of RNA silencing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.