ArticlePlant molecular biology2005
Identification of a novel cis-element exhibiting cytokinin-dependent protein binding in vitro in the 5'-region of NADPH-protochlorophyllide oxidoreductase gene in cucumber.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.
- Identification of cytokinin-responsive genes using microarray meta-analysis and RNA-Seq in Arabidopsis.Plant physiology · 2013Pooled it
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- Combined strategy employing MutMap and RNA-seq reveals genomic regions and genes associated with complete panicle exsertion in rice.Molecular breeding : new strategies in plant improvement · 2023Article
- Genome-wide identification and expression profiling of two-component system (TCS) genes inFrontiers in genetics · 2023Article
- The chloroplast redox-responsive transcriptome of solanaceous plants reveals significant nuclear gene regulatory motifs associated to stress acclimation.Plant molecular biology · 2022Article
- Cloning and functional characterization of seed-specific LEC1A promoter from peanut (Arachis hypogaea L.).PloS one · 2021Article
- Allelic diversity of phytoene synthase gene influences the transcription level in citrus fruit among a citrus FBreeding science · 2017Article
- In silico analysis of 3 expansin gene promoters reveals 2 hubs controlling light and cytokinins response during bud outgrowth.Plant signaling & behavior · 2017Article
- Distinctive features and differential regulation of the DRTS genes of Arabidopsis thaliana.PloS one · 2017Article
- Cis-Regulatory Elements Determine Germline Specificity and Expression Level of an Isopentenyltransferase Gene in Sperm Cells of Arabidopsis.Plant physiology · 2016Article
- The Grapevine VvPMEI1 Gene Encodes a Novel Functional Pectin Methylesterase Inhibitor Associated to Grape Berry Development.PloS one · 2015Article
- Cloning and Characterization of 5' Flanking Regulatory Sequences of AhLEC1B Gene from Arachis Hypogaea L.PloS one · 2015Article
- Genome-wide identification, phylogeny, duplication, and expression analyses of two-component system genes in Chinese cabbage (Brassica rapa ssp. pekinensis).DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
- Deep transcriptome sequencing of rhizome and aerial-shoot in Sorghum propinquum.Plant molecular biology · 2014Article
- A root-specific wall-associated kinase gene, HvWAK1, regulates root growth and is highly divergent in barley and other cereals.Functional & integrative genomics · 2013Article
- Tissue-specific transcriptomic profiling of Sorghum propinquum using a rice genome array.PloS one · 2013Article
- Molecular cloning and functional characterization of Catharanthus roseus hydroxymethylbutenyl 4-diphosphate synthase gene promoter from the methyl erythritol phosphate pathway.Molecular biology reports · 2012Article
- Gene regulation by cytokinin in Arabidopsis.Frontiers in plant science · 2012Article
- Conservation and divergence of autonomous pathway genes in the flowering regulatory network of Beta vulgaris.Journal of experimental botany · 2011Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinins and light activate the transcription of the cucumber NADPH-protochlorophyllide reductase (POR) gene. We have previously reported that 2.3 kb of the 5'-region of this gene contains a cis-element that is responsive to cytokinin. In this study, to identify the cytokinin-responsive cis-element corresponding to chlorophyll biosynthesis and chloroplast development, we performed transient expression assays in etiolated cucumber cotyledons. A 5'-deletional analysis indicated that a 411-bp fragment (-451 to -40 bp) contained at least one of the cis-elements related to cytokinin-responsiveness. Gel mobility shift assays also detected cytokinin-enhanced binding in this region. DNase I footprinting analysis, using a 150-bp fragment (-490 to -340 bp) as the probe, identified the cytokinin-enhanced protected sequence as 5'-ATATTAGTGATAT-3'. More detailed gel mobility shift and competition analyses identified 5'-TATTAG-3' as the sequence critical for cytokinin-enhanced binding. Mutations in the identified sequence in the transient expression assay caused a reduced but retained cytokinin-responsiveness, as well as low reporter activity of untreated control. These results suggest that the identified sequence is a novel cis-element exhibiting cytokinin-dependent protein binding in vitro, which may function effectively when interacting with other cytokinin-related elements. The effects of this element on the chloroplast development are discussed in relation to other cytokinin-related elements.
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