ArticlePlant molecular biology2005
Active RNA silencing at low temperature indicates distinct pathways for antisense-mediated gene-silencing in potato.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 24 citations in OpenAlex.
- Structural and Functional Characterization of the VQ Protein Family and VQ Protein Variants from Soybean.Scientific reports · 2016Article
- Light intensity and temperature affect systemic spread of silencing signal in transient agroinfiltration studies.Molecular plant pathology · 2015Article
- RNA interference with special reference to combating viruses of crustacea.Indian journal of virology : an official organ of Indian Virological Society · 2012Article
- Suppressive mechanism of seed coat pigmentation in yellow soybean.Breeding science · 2012Article
- Variation of GmIRCHS (Glycine max inverted-repeat CHS pseudogene) is related to tolerance of low temperature-induced seed coat discoloration in yellow soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2011Article
- Light intensity affects RNA silencing of a transgene in Nicotiana benthamiana plants.BMC plant biology · 2010Article
- Efficient and stable transgene suppression via RNAi in field-grown poplars.Transgenic research · 2008Article
- Transgenic resistance to Citrus tristeza virus in grapefruit.Plant cell reports · 2008Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, it was shown that low temperature (<or= 15 degrees C) inhibits RNA silencing-mediated defence by the control of siRNA generation. In contrast, we have found nine antisense potato lines out of 24 in which RNA silencing was not inhibited at low temperature. In these lines, the extent of endogenous repression varied in leaves and was found to be different in roots and tubers. In order to address the contribution of gene dosage and repetitive structure of the transgene loci to the temperature dependence/independence of antisense-mediated gene-silencing DNA gel blot analysis was performed. Interestingly, none of the studied features correlated with the observed silencing effect. In addition, the insertion of vector backbone sequences into the potato genome did not influence the temperature dependence. RNA-directed DNA methylation was detected in the majority of antisense lines, however, it was also independent of the type of RNA silencing. Thus, it is feasible that chromosomal flanking sequences or the chromatin structure surrounding the insertion determine which silencing pathway is activated.
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