ArticlePlant molecular biology2005
Expression patterns of purple acid phosphatase genes in Arabidopsis organs and functional analysis of AtPAP23 predominantly transcribed in flower.
Article in Plant molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 94 citations in OpenAlex.
- Uncovering adaptation with a new Arabidopsis thaliana multiparent intercross population.Genetics · 2026Article
- Genome-wide identification, characterization and expression profiling of purple acid phosphatases under variable phosphorus regimes in lentil (Lens culinaris Medik.).BMC plant biology · 2025Article
- Vacuolar phosphatases are essential for efficient nucleotide salvage in Arabidopsis.Journal of experimental botany · 2025Article
- Genome-Wide Analysis, Identification, and Transcriptional Profile of the Response to Abiotic Stress of theInternational journal of molecular sciences · 2025Article
- TheInternational journal of molecular sciences · 2024Article
- Functional assessment of AtPAP17; encoding a purple acid phosphatase involved in phosphate metabolism in Arabidopsis thaliana.Biotechnology letters · 2023Article
- Narrowing down molecular targets for improving phosphorus-use efficiency in maize (Zea mays L.).Molecular biology reports · 2022Review
- Purple acid phosphatases in coffee-genome-wide identification and the transcriptional profile of selected members in roots upon Pi starvation.3 Biotech · 2022Article
- Fine-tuning the transcriptional regulatory model of adaptation response to phosphate stress in maize (Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Structure of a cereal purple acid phytase provides new insights to phytate degradation in plants.Plant communications · 2022Article
- The PAP Gene Family in Tomato: Comprehensive Comparative Analysis, Phylogenetic Relationships and Expression Profiles.Plants (Basel, Switzerland) · 2022Article
- Transcriptome analysis provides new insights into plants responses under phosphate starvation in association with chilling stress.BMC plant biology · 2022Article
- Purple acid phosphatases: roles in phosphate utilization and new emerging functions.Plant cell reports · 2022Review
- Building a Flower: The Influence of Cell Wall Composition on Flower Development and Reproduction.Genes · 2021Review
- Transcriptome Analysis ofFrontiers in plant science · 2021Article
- Globoids and Phytase: The Mineral Storage and Release System in Seeds.International journal of molecular sciences · 2020Review
- Identification of Tea Plant Purple Acid Phosphatase Genes and Their Expression Responses to Excess Iron.International journal of molecular sciences · 2019Article
- Genome-wide analysis of purple acid phosphatase structure and expression in ten vegetable species.BMC genomics · 2018Article
- Article
- Functional Assessment of an Overexpressed Arabidopsis Purple Acid Phosphatase Gene (Iranian journal of biotechnology · 2018Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purple acid phosphatases (PAPs) are metallo-phosphoesterases. Their expression and function have not been systematically investigated in higher plants. In this work, we compared the transcript levels of 28 Arabidopsis PAP (AtPAP) genes in five Arabidopsis organs. The 28 members, although differed in their expression patterns in vegetative organs, were all transcribed in flower. Furthermore, the transcription of seven members (AtPAPs 6, 11, 14, 19, 23, 24 and 25) occurred predominantly in the flower. To begin dissecting the role of AtPAP genes in flower development, further expression and functional analyses were conducted using AtPAP23. Histochemical staining of transgenic plants expressing AtPAP23 promoter-beta-glucuronidase (GUS) gene construct revealed that AtPAP23 transcription was strong in flower apical meristems, but became restricted to petals and anther filaments in fully developed flower. A GST (glutathione S-transferase) fusion protein of AtPAP23 (GST:AtPAP23) was expressed in bacterial cells, and was found to contain significant amounts of Fe and Mn (whereas the control GST protein contained none). In biochemical tests, GST:AtPAP23 showed typical acid phosphatase activities. The fusion protein was also highly active on phosphoserine, but not phosphotyrosine. Despite its highly specific expression pattern and the demonstrated biochemical function of its protein product, the RNAi (RNA interference), T-DNA knock-out and overexpression lines of AtPAP23 were indistinguishable from wild type plants in the development of flower (or other organs). Interestingly, the Fe and Mn contents were found significantly increased in AtPAP23 overexpression lines, which may offer a new direction for further functional studies of AtPAPs in Arabidopsis.
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