Evidence map›Paper›PMID 16244899›Full record

ArticleMycopathologia2005

Differential expression of chitin synthase (CHS) and glucan synthase (FKS) genes correlates with the formation of a modified, thinner cell wall in in vivo-produced Beauveria bassiana cells.

Aurélien Tartar, Alexandra M Shapiro, Dancia W Scharf, Drion G Boucias

Abstract read
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In one paragraph

Article in Mycopathologia, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Frontiers in fungal biology · 2022
    Review
  7. Article
  8. Spent Brewer's Yeast as a Source of Insoluble β-Glucans.International journal of molecular sciences · 2021
    Review
  9. Review
  10. Article
  11. Article
  12. Article
  13. Review
  14. Prophenoloxidase-MediatedFrontiers in immunology · 2017
    Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Aurélien TartarDepartment of Biology, Clark University, Worcester, MA 01610, USA.
Alexandra M Shapiro
Dancia W Scharf
Drion G Boucias

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During infection (in vivo), the entomopathogenic fungus Beauveria bassiana produces yeast-like cells that are surrounded by modified cell walls. These modifications have been related to the fungus ability to limit recognition by the host defense system. The composition of the in vivo cell wall was analyzed using a combination of cytochemical and molecular techniques. The in vivo cell walls still contained both chitin and 1,3-beta-glucan, but they were significantly thinner than in vitro cell walls (50-60 nm versus 100-160 nm, respectively). The difference in cell wall thickness was correlated with transcriptional regulation of cell wall-related genes: quantitative RT-PCR reactions demonstrated that B. bassiana chitin synthase (CHS) and glucan synthase (FKS) genes are down regulated in vivo. These analyses indicate that in vivo-triggered phenotypic modifications, including cell wall adjustments, are controlled by molecular mechanisms that include regulation of gene expression at the transcriptional level.

Indexed as

Amino Acid SequenceAnimalsBase Sequencebeta-GlucansCell WallChitinChitin SynthaseDNA, FungalFungiGlucosyltransferasesInsectaMicroscopy, Electron, TransmissionMicroscopy, FluorescencePhylogenyRandom Amplified Polymorphic DNA TechniqueReverse Transcriptase Polymerase Chain Reactionbeta-1,3-glucanbeta-GlucansChitinChitin SynthaseDNA, Fungalglucan synthaseGlucosyltransferases

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.