ArticlePlant physiology2005
Identification of three urease accessory proteins that are required for urease activation in Arabidopsis.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 76 citations in OpenAlex.
- Unveiling the role of microbial rease in ureolysis-induced calcium carbonate precipitation, Its mechanistic insights, and emerging applications.World journal of microbiology & biotechnology · 2025Review
- Article
- Two related families of metal transferases, ZNG1 and ZNG2, are involved in acclimation to poor Zn nutrition in Arabidopsis.Frontiers in plant science · 2023Article
- The role of symbiotic fungi in the life cycle ofFrontiers in plant science · 2023Review
- Literature Review on the Effects of Heavy Metal Stress and Alleviating Possibilities through Exogenously Applied Agents in Alfalfa (Plants (Basel, Switzerland) · 2022Review
- Broad-range metalloprotease profiling in plants uncovers immunity provided by defence-related metalloenzyme.The New phytologist · 2022Article
- Identification and Fine Mapping of the Candidate Gene Controlling Multi-Inflorescence inInternational journal of molecular sciences · 2022Article
- Indigenous Microorganisms Offset Arbuscular Mycorrhizal Fungi-Induced Plant Growth and Nutrient Acquisition Through Negatively Modulating the Genes of Phosphorus Transport and Nitrogen Assimilation.Frontiers in plant science · 2022Article
- A Kinase and a Glycosylase Catabolize Pseudouridine in the Peroxisome to Prevent Toxic Pseudouridine Monophosphate Accumulation.The Plant cell · 2020Article
- Oomycete Gene Table: an online database for comparative genomic analyses of the oomycete microorganisms.Database : the journal of biological databases and curation · 2019Article
- mThe Plant cell · 2018Article
- The Gastrodia elata genome provides insights into plant adaptation to heterotrophy.Nature communications · 2018Article
- Biochemical and genetic analyses of the oomycetePeerJ · 2018Article
- Coprophagous features in carnivorous Nepenthes plants: a task for ureases.Scientific reports · 2017Article
- The assembly of the plant urease activation complex and the essential role of the urease accessory protein G (UreG) in delivery of nickel to urease.The Journal of biological chemistry · 2017Article
- Biochemical and genetic analyses of N metabolism in maize testcross seedlings: 1. Leaves.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- Glutaredoxin GRXS17 Associates with the Cytosolic Iron-Sulfur Cluster Assembly Pathway.Plant physiology · 2016Article
- Of the Nine Cytidine Deaminase-Like Genes in Arabidopsis, Eight Are Pseudogenes and Only One Is Required to Maintain Pyrimidine Homeostasis in Vivo.Plant physiology · 2016Article
- Urea retranslocation from senescing Arabidopsis leaves is promoted by DUR3-mediated urea retrieval from leaf apoplast.The Plant journal : for cell and molecular biology · 2015Article
- Physiological implications of arginine metabolism in plants.Frontiers in plant science · 2015Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Urease is a nickel-containing urea hydrolase involved in nitrogen recycling from ureide, purine, and arginine catabolism in plants. The process of urease activation by incorporation of nickel into the active site is a prime example of chaperone-mediated metal transfer to an enzyme. Four urease accessory proteins are required for activation in Klebsiella aerogenes. In plants urease accessory proteins have so far been only partially defined. Using reverse genetic tools we identified four genes that are necessary for urease activity in Arabidopsis (Arabidopsis thaliana; ecotypes Columbia and Nössen). Plants bearing T-DNA or Ds element insertions in either the structural gene for urease or in any of the three putative urease accessory genes AtureD, AtureF, and AtureG lacked the corresponding mRNAs and were defective in urease activity. In contrast to wild-type plants, the mutant lines were not able to support growth with urea as the sole nitrogen source. To investigate whether the identified accessory proteins would be sufficient to support eukaryotic urease activation, the corresponding cDNAs were introduced into urease-negative Escherichia coli. In these bacteria, urease activity was observed only when all three plant accessory genes were coexpressed together with the plant urease gene. Remarkably, plant urease activation occurred as well in cell-free E. coli extracts, but only in extracts from cells that had expressed all three accessory proteins. The future molecular dissection of the plant urease activation process may therefore be performed in vitro, providing a powerful tool to further our understanding of the biochemistry of chaperone-mediated metal transfer processes in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.