ArticleThe Plant cell2005
Functional differentiation of bundle sheath and mesophyll maize chloroplasts determined by comparative proteomics.
Article in The Plant cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers, 1 of them a synthesis that pooled it.
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Who cites it
92 citing papers in PubMed, 1 synthesis or guideline pooled it, 244 citations in OpenAlex.
- Freeze-quenched maize mesophyll and bundle sheath separation uncovers bias in previous tissue-specific RNA-Seq data.Journal of experimental botany · 2017Pooled it
- Vestigial Plastids in Parasitic Plants: Evolutionary Remnants or Adaptive Innovations?Plant, cell & environment · 2026Review
- Exploring the diversity of the CO2-concentrating mechanism in different C4 subtypes.Journal of experimental botany · 2026Article
- Bundle sheath cell-specific expression of chloroplast genes encoding subunits of the NADH dehydrogenase-like complex in maize.The Plant journal : for cell and molecular biology · 2025Article
- Regulation of Crassulacean acid metabolism at the protein level in Kalanchoë laxiflora.Plant physiology · 2025Article
- Identification of Two Flip-Over Genes in Grass Family as Potential Signature of C4 Photosynthesis Evolution.International journal of molecular sciences · 2023Article
- Comparison of plastid proteomes points towards a higher plastidial redox turnover in vascular tissues than in mesophyll cells.Journal of experimental botany · 2023Article
- Assessment of the Photosynthetic Apparatus Functions by Chlorophyll Fluorescence and PInternational journal of molecular sciences · 2022Article
- Article
- Chloroplastic photoprotective strategies differ between bundle sheath and mesophyll cells in maize (Frontiers in plant science · 2022Article
- Ribosome profiling elucidates differential gene expression in bundle sheath and mesophyll cells in maize.Plant physiology · 2021Article
- Installation of CPlant biotechnology journal · 2021Article
- Article
- Autophagy Plays Prominent Roles in Amino Acid, Nucleotide, and Carbohydrate Metabolism during Fixed-Carbon Starvation in Maize.The Plant cell · 2020Article
- Genome-Scale Characterization of Predicted Plastid-Targeted Proteomes in Higher Plants.Scientific reports · 2020Article
- Green leaf volatiles and jasmonic acid enhance susceptibility to anthracnose diseases caused by Colletotrichum graminicola in maize.Molecular plant pathology · 2020Article
- Cytological evidence of BSD2 functioning in both chloroplast division and dimorphic chloroplast formation in maize leaves.BMC plant biology · 2020Article
- Insights Into the Regulation of the Expression Pattern of Calvin-Benson-Bassham Cycle Enzymes in CFrontiers in plant science · 2020Article
- MaizeThe Journal of cell biology · 2019Article
- Application of Proteomics Technologies in Oil Palm Research.The protein journal · 2018Review
32 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chloroplasts of maize (Zea mays) leaves differentiate into specific bundle sheath (BS) and mesophyll (M) types to accommodate C4 photosynthesis. Consequences for other plastid functions are not well understood but are addressed here through a quantitative comparative proteome analysis of purified M and BS chloroplast stroma. Three independent techniques were used, including cleavable stable isotope coded affinity tags. Enzymes involved in lipid biosynthesis, nitrogen import, and tetrapyrrole and isoprenoid biosynthesis are preferentially located in the M chloroplasts. By contrast, enzymes involved in starch synthesis and sulfur import preferentially accumulate in BS chloroplasts. The different soluble antioxidative systems, in particular peroxiredoxins, accumulate at higher levels in M chloroplasts. We also observed differential accumulation of proteins involved in expression of plastid-encoded proteins (e.g., EF-Tu, EF-G, and mRNA binding proteins) and thylakoid formation (VIPP1), whereas others were equally distributed. Enzymes related to the C4 shuttle, the carboxylation and regeneration phase of the Calvin cycle, and several regulators (e.g., CP12) distributed as expected. However, enzymes involved in triose phosphate reduction and triose phosphate isomerase are primarily located in the M chloroplasts, indicating that the M-localized triose phosphate shuttle should be viewed as part of the BS-localized Calvin cycle, rather than a parallel pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.