ArticleAmerican journal of respiratory cell and molecular biology2006
Dexamethasone-mediated repression of MUC5AC gene expression in human lung epithelial cells.
Article in American journal of respiratory cell and molecular biology, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 76 citations in OpenAlex.
- Glucocorticoid therapy in respiratory illness: bench to bedside.Journal of investigative medicine : the official publication of the American Federation for Clinical Research · 2022Review
- Epithelial Barrier Dysfunction in Chronic Respiratory Diseases.Frontiers in physiology · 2021Review
- Chemocentric Informatics Analysis: DexamethasoneACS omega · 2020Article
- Expression and Clinical Significance of Mucin Gene in Chronic Rhinosinusitis.Current allergy and asthma reports · 2020Review
- Strategies for measuring airway mucus and mucins.Respiratory research · 2019Review
- Characterization of the human mucin 5AC promoter and its regulation by the histone acetyltransferase P300.International journal of molecular medicine · 2019Article
- Reconstituting Mouse Lungs with Conditionally Reprogrammed Human Bronchial Epithelial Cells.Tissue engineering. Part A · 2018Article
- Birth delivery method affects expression of immune genes in lung and jejunum tissue of neonatal beef calves.BMC veterinary research · 2017Article
- IL-4-induced caveolin-1-containing lipid rafts aggregation contributes to MUC5AC synthesis in bronchial epithelial cells.Respiratory research · 2017Article
- Mometasone Furoate Suppresses PMA-Induced MUC-5AC and MUC-2 Production in Human Airway Epithelial Cells.Tuberculosis and respiratory diseases · 2017Article
- Fenofibrate Attenuates Neutrophilic Inflammation in Airway Epithelia: Potential Drug Repurposing for Cystic Fibrosis.Clinical and translational science · 2015Article
- IL-1β induction of MUC5AC gene expression is mediated by CREB and NF-κB and repressed by dexamethasone.American journal of physiology. Lung cellular and molecular physiology · 2014Article
- Clinical issues of mucus accumulation in COPD.International journal of chronic obstructive pulmonary disease · 2014Review
- Chronic bronchitis and chronic obstructive pulmonary disease.American journal of respiratory and critical care medicine · 2013Review
- Lipopolysaccharides induces MUC5AC overproduction in human nasal epithelium.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2013Article
- CFTR, mucins, and mucus obstruction in cystic fibrosis.Cold Spring Harbor perspectives in medicine · 2012Review
- AGR2 is induced in asthma and promotes allergen-induced mucin overproduction.American journal of respiratory cell and molecular biology · 2012Article
- Human bronchial epithelial cells differentiate to 3D glandular acini on basement membrane matrix.American journal of respiratory cell and molecular biology · 2011Article
- [Effect of dexamethasone on cisplatin induced apoptosis in primary cells from resected specimens with Chinese human lung adenocarcinoma ex vivo].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2010Article
- Regulation of airway MUC5AC expression by IL-1beta and IL-17A; the NF-kappaB paradigm.Journal of immunology (Baltimore, Md. : 1950) · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Glucocorticoids regulate gene expression via binding of the ligand-activated glucocorticoid receptor (GR) to glucocorticoid-responsive elements (GRE) in target gene promoters. The MUC5AC gene, which encodes the protein backbone of an abundant secreted airway mucin, has several putative GRE cis-elements in its 5' sequence. Mechanism(s) whereby glucocorticoids regulate mucin genes have not previously been described. In this study, the glucocorticoid dexamethasone (Dex) decreased MUC5AC mRNA abundance in A549 and NCI-H292 cell lines and primary differentiated normal bronchial epithelial cells by 50-80%, suggesting a common mechanism of MUC5AC gene repression in human lung epithelial cells. Kinetic analyses showed that MUC5AC mRNA was not significantly decreased until 6 h after Dex exposure, and that nuclear translocation of GR was biphasic, suggesting that Dex-mediated cis-repression of MUC5AC gene expression was a delayed response of GR translocation. Transfection analyses demonstrated that Dex transcriptionally repressed the MUC5AC promoter. Electrophoretic mobility shift assays with wild-type and mutant oligonucleotide probes showed that GR bound to two GRE cis-sites (nucleotides -930 to -912 and -369 to -351) in the MUC5AC promoter. Analyses of mutated MUC5AC promoter constructs demonstrated that NF-kappaB cis-sites were not involved in Dex-mediated repression of MUC5AC. Dex did not alter mRNA stability of MUC5AC transcripts. Taken together, the data indicate that Dex transcriptionally mediates repression of MUC5AC gene expression in human lung epithelial cells at quiescent states after binding of GR to one or more GRE cis-elements in the MUC5AC promoter.
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