ArticleInfection and immunity2005
Analysis of a heme-dependent signal transduction system in Corynebacterium diphtheriae: deletion of the chrAS genes results in heme sensitivity and diminished heme-dependent activation of the hmuO promoter.
Article in Infection and immunity, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 34 citations in OpenAlex.
- Regulation of Ansamitocin P-3 Biosynthesis by the Response Regulator CrsB in Actinosynnema pretiosum.Current microbiology · 2026Article
- HrrSA orchestrates a systemic response to heme and determines prioritization of terminal cytochrome oxidase expression.Nucleic acids research · 2020Article
- Transcriptome profile of Corynebacterium pseudotuberculosis in response to iron limitation.BMC genomics · 2019Article
- Corynebacterium diphtheriae Iron-Regulated Surface Protein HbpA Is Involved in the Utilization of the Hemoglobin-Haptoglobin Complex as an Iron Source.Journal of bacteriology · 2018Article
- Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.Microbiology and molecular biology reviews : MMBR · 2017Review
- Structure of the response regulator ChrA in the haem-sensing two-component system of Corynebacterium diphtheriae.Acta crystallographica. Section F, Structural biology communications · 2015Article
- The crimson conundrum: heme toxicity and tolerance in GAS.Frontiers in cellular and infection microbiology · 2014Article
- Two-component signal transduction in Corynebacterium glutamicum and other corynebacteria: on the way towards stimuli and targets.Applied microbiology and biotechnology · 2012Review
- The ChrA response regulator in Corynebacterium diphtheriae controls hemin-regulated gene expression through binding to the hmuO and hrtAB promoter regions.Journal of bacteriology · 2012Article
- Extracellular heme uptake and the challenges of bacterial cell membranes.Current topics in membranes · 2012Article
- Control of heme homeostasis in Corynebacterium glutamicum by the two-component system HrrSA.Journal of bacteriology · 2011Article
- Overcoming the heme paradox: heme toxicity and tolerance in bacterial pathogens.Infection and immunity · 2010Review
- The ABC transporter HrtAB confers resistance to hemin toxicity and is regulated in a hemin-dependent manner by the ChrAS two-component system in Corynebacterium diphtheriae.Journal of bacteriology · 2010Article
- Structure and heme binding properties of Escherichia coli O157:H7 ChuX.Protein science : a publication of the Protein Society · 2009Article
- Regulation and activity of a zinc uptake regulator, Zur, in Corynebacterium diphtheriae.Journal of bacteriology · 2009Article
- The role of the cytoplasmic heme-binding protein (PhuS) of Pseudomonas aeruginosa in intracellular heme trafficking and iron homeostasis.The Journal of biological chemistry · 2009Article
- The heme sensor system of Staphylococcus aureus.Contributions to microbiology · 2009Review
- The ChrA-ChrS and HrrA-HrrS signal transduction systems are required for activation of the hmuO promoter and repression of the hemA promoter in Corynebacterium diphtheriae.Infection and immunity · 2007Article
- Comparative analysis of hmuO function and expression in Corynebacterium species.Journal of bacteriology · 2007Article
- Staphylococcus aureus redirects central metabolism to increase iron availability.PLoS pathogens · 2006Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Corynebacterium diphtheriae hmuO gene encodes a heme oxygenase that is involved in the utilization of heme as an iron source. Transcription of hmuO is activated by heme or hemoglobin and repressed by iron and DtxR. Previous studies with Escherichia coli showed that heme-dependent transcriptional activation of an hmuO promoter-lacZ fusion was dependent on the cloned C. diphtheriae chrA and chrS genes (chrAS), which encode the response regulator and sensor kinase, respectively, of a two-component signal transduction system. In this study, nonpolar deletions in the chrAS genes were constructed on the chromosome of C. diphtheriae. Mutations in chrAS resulted in marked reduction in heme-dependent transcription of hmuO, which indicates that the ChrA/S system is a key regulator at the hmuO promoter. However, low but significant levels of heme-specific transcriptional activity were observed at the hmuO promoter in the chrAS mutants, suggesting that an additional heme-dependent activator is involved in hmuO expression. The chrAS mutants were also sensitive to heme, which was observed only in stationary-phase cultures and correlated with reduced cell viability. The heme sensitivity of the mutants was not due to reduced expression of hmuO, and these results suggest that additional factors controlled by the ChrA/S system may be involved in protection against heme toxicity. Transcriptional analysis of the chrAS operon revealed that it was not autoregulated or affected by iron or heme levels.
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