ArticleThe EMBO journal2005
Microtubule-dependent transport and organization of sarcomeric myosin during skeletal muscle differentiation.
Article in The EMBO journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
43 citing papers in PubMed.
- Interplay between microtubule interactome, myonuclei mechanotransduction, and positioning in myopathies.Nucleus (Austin, Tex.) · 2025Review
- Architecture and molecular machinery of skeletal myofibers: a systematic review of the structure-function relationships.Frontiers in cell and developmental biology · 2025Review
- Filamin-A-interacting protein 1 (FILIP1) is a dual compartment protein linking myofibrils and microtubules during myogenic differentiation and upon mechanical stress.Cell and tissue research · 2023Article
- Insights into Cell-Specific Functions of Microtubules in Skeletal Muscle Development and Homeostasis.International journal of molecular sciences · 2023Review
- The myonuclear domain in adult skeletal muscle fibres: past, present and future.The Journal of physiology · 2023Review
- Detyrosinated microtubule arrays drive myofibrillar malformations inFrontiers in cell and developmental biology · 2023Article
- The circadian E3 ligase FBXL21 regulates myoblast differentiation and sarcomere architecture via MYOZ1 ubiquitination and NFAT signaling.PLoS genetics · 2022Article
- Development of the indirect flight muscles of Aedes aegypti, a main arbovirus vector.BMC developmental biology · 2021Article
- Nuclear Mechanotransduction in Skeletal Muscle.Cells · 2021Review
- The actin polymerization factor Diaphanous and the actin severing protein Flightless I collaborate to regulate sarcomere size.Developmental biology · 2021Article
- Mdfi Promotes C2C12 Cell Differentiation and Positively Modulates Fast-to-Slow-Twitch Muscle Fiber Transformation.Frontiers in cell and developmental biology · 2021Article
- Computational Assessment of Transport Distances in Living Skeletal Muscle Fibers Studied In Situ.Biophysical journal · 2020Article
- 17β-estradiol alters mRNA co-expression after murine muscle injury and mild hypobaria.Experimental biology and medicine (Maywood, N.J.) · 2019Article
- Persistent upregulation of the β-tubulin tubb6, linked to muscle regeneration, is a source of microtubule disorganization in dystrophic muscle.Human molecular genetics · 2019Article
- Review
- Identification of cardioprotective drugs by medium-scaleDisease models & mechanisms · 2018Article
- Small molecule screen in embryonic zebrafish using modular variations to target segmentation.Nature communications · 2017Article
- TheeLife · 2016Article
- Muscle RING-finger 2 and 3 maintain striated-muscle structure and function.Journal of cachexia, sarcopenia and muscle · 2016Article
- Homologous Transcription Factors DUX4 and DUX4c Associate with Cytoplasmic Proteins during Muscle Differentiation.PloS one · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It has been proposed that microtubules (MTs) participate in skeletal muscle cell differentiation. However, it is still unclear how this happens. To examine whether MTs could participate directly in the organization of thick and thin filaments into sarcomeres, we observed the concomitant reorganization and dynamics of MTs with the behavior of sarcomeric actin and myosin by time-lapse confocal microscopy. Using green fluorescent protein (GFP)-EB1 protein to label MT plus ends, we determined that MTs become organized into antiparallel arrays along fusing myotubes. Their dynamics and orientation was found to be different across the thickness of the myotubes. We observed fast movements of Dsred-myosin along GFP-MTs. Comparison of GFP-EB1 and Dsred-myosin dynamics revealed that myosin moved toward MT plus ends. Immuno-electron microscopy experiments confirmed that myosin was actually associated with MTs in myotubes. Finally, we confirmed that MTs were required for the stabilization of myosin-containing elements prior to incorporation into mature sarcomeres. Collectively, our results strongly suggest that MTs become organized into a scaffold that provides directional cues for the positioning and organization of myosin filaments during sarcomere formation.
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