ArticleMicrobiology and immunology2005
In silico analysis of ORF1ab in coronavirus HKU1 genome reveals a unique putative cleavage site of coronavirus HKU1 3C-like protease.
Article in Microbiology and immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 40 citations in OpenAlex.
- Proteolytic Processing of the Coronavirus Replicase Nonstructural Protein 14 Exonuclease Is Not Required for Virus Replication but Alters RNA Synthesis and Viral Fitness.Journal of virology · 2022Article
- SARS-CoV-2 3CLpro whole human proteome cleavage prediction and enrichment/depletion analysis.Computational biology and chemistry · 2022Article
- Correlated sequence signatures are present within the genomic 5'UTR RNA and NSP1 protein in coronaviruses.RNA (New York, N.Y.) · 2022Article
- Screening of Severe Acute Respiratory Syndrome Coronavirus 2 RNA-Dependent RNA Polymerase Inhibitors Using Computational Approach.Journal of computational biology : a journal of computational molecular cell biology · 2021Article
- High-throughput analysis of the interactions between viral proteins and host cell RNAs.Computers in biology and medicine · 2021Article
- FDA-approved thiol-reacting drugs that potentially bind into the SARS-CoV-2 main protease, essential for viral replication.Journal of biomolecular structure & dynamics · 2021Article
- Application of Tensor Decomposition to Gene Expression of Infection of Mouse Hepatitis Virus Can Identify Critical Human Genes and Efffective Drugs for SARS-CoV-2 Infection.IEEE journal of selected topics in signal processing · 2021Article
- Analysis of SARS-CoV-2 RNA-dependent RNA polymerase as a potential therapeutic drug target using a computational approach.Journal of translational medicine · 2020Article
- Article
- Middle East respiratory syndrome coronavirus: another zoonotic betacoronavirus causing SARS-like disease.Clinical microbiology reviews · 2015Review
- Human coronaviruses: Clinical features and phylogenetic analysis.BioMedicine · 2013Review
- Characterization of Bafinivirus main protease autoprocessing activities.Journal of virology · 2011Article
- Coronavirus genomics and bioinformatics analysis.Viruses · 2010Article
- Functional and genetic studies of the substrate specificity of coronavirus infectious bronchitis virus 3C-like proteinase.Journal of virology · 2010Article
- More and More Coronaviruses: Human Coronavirus HKU1.Viruses · 2009Article
- Expression, crystallization and preliminary crystallographic study of human coronavirus HKU1 nonstructural protein 9.Acta crystallographica. Section F, Structural biology and crystallization communications · 2009Article
- Comparative analysis of complete genome sequences of three avian coronaviruses reveals a novel group 3c coronavirus.Journal of virology · 2009Article
- Structure of the main protease from a global infectious human coronavirus, HCoV-HKU1.Journal of virology · 2008Article
- CoVDB: a comprehensive database for comparative analysis of coronavirus genes and genomes.Nucleic acids research · 2008Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recently we have described the discovery and complete genome sequence of a novel coronavirus associated with pneumonia, coronavirus HKU1 (CoV-HKU1). In this study, a detailed in silico analysis of the ORF1ab, encoding the 7,182-amino acid replicase polyprotein in the CoV-HKU1 genome showed that the replicase polyprotein of CoV-HKU1 is cleaved by its papain-like proteases and 3C-like protease (3CL(pro)) into 16 polypeptides homologous to the corresponding polypeptides in other coronaviruses. Surprisingly, analysis of the putative cleavage sites of the 3CL(pro) revealed a unique putative cleavage site. In all known coronaviruses, the P1 positions at the cleavage sites of the 3CL(pro) are occupied by glutamine. This is also observed in CoV-HKU1, except for one site at the junction between nsp10 (helicase) and nsp11 (member of exonuclease family), where the P1 position is occupied by histidine. This amino acid substitution is due to a single nucleotide mutation in the CoV-HKU1 genome, CAG/A to CAT. This probably represents a novel cleavage site because the same mutation was consistently observed in CoV-HKU1 sequences from multiple specimens of different patients; the P2 and P1'-P12' positions of this cleavage site are consistent between CoV-HKU1 and other coronaviruses; and as the helicase is one of the most conserved proteins in coronaviruses, cleavage between nsp10 and nsp11 should be an essential step for the generation of the mature functional helicase. Experiments, including purification and C-terminal amino acid sequencing of the CoV-HKU1 helicase and trans-cleavage assays of the CoV-HKU1 3CL(pro) will confirm the presence of this novel cleavage site.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.