ArticleThe Journal of molecular diagnostics : JMD2005
Detection of medically important Ehrlichia by quantitative multicolor TaqMan real-time polymerase chain reaction of the dsb gene.
Article in The Journal of molecular diagnostics : JMD, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 266 citations in OpenAlex.
- Rapid Gel-Based Recombinase Polymerase Amplification Assay for Detection and Sequence Confirmation ofAnimals : an open access journal from MDPI · 2026Article
- Molecular detection of Ehrlichia canis and Rickettsia rickettsii in tick-infested dogs from the city of Chihuahua, Mexico: phylogenetic analysis of the gp36 and ompA genes.Veterinary research communications · 2026Article
- Molecular detection and phylogenetic characterization of Ehrlichia- and Anaplasma-related genotypes in free-ranging anteaters from southeastern Brazil.Veterinary research communications · 2026Article
- Molecular detection and phylogenetic characterization of Babesia and Ehrlichia in free-living opossums from southeastern Brazil.Acta parasitologica · 2026Article
- Occurrence, Risk Factors, and Molecular Characterization ofVeterinary sciences · 2026Article
- A survey of vector-borne bacteria in bat-associated mites (Mesostigmata: Macronyssidae and Spinturnicidae) from Brazil reveals the presence of Ehrlichia spp.Veterinary research communications · 2026Article
- Molecular-based detection of Ehrlichia spp. in stray dogs-infesting Rhipicephalus ticks in high-altitude of northern Pakistan.PloS one · 2026Article
- Molecular Survey of Hemopathogens in Dogs, Including Blood Donors, from Central-Western Brazil.Pathogens (Basel, Switzerland) · 2025Article
- A multidimensional analysis of the risk of infection with Ehrlichia canis among urban dogs in Iquitos, Peru.Scientific reports · 2025Article
- Seach for tick-borne agents and detection of a novel Ehrlichia sp. in ticks from wild rabbits in Northern Spain.Experimental & applied acarology · 2025Article
- Molecular survey of Anaplasmataceae agents, piroplasmids and haemosporidians in seabirds (Procellariiformes and Sphenisciformes) rescued on the Brazilian coast.Veterinary research communications · 2025Article
- Molecular Survey of Hemopathogens in Bats from the Western Brazilian Amazon.Pathogens (Basel, Switzerland) · 2025Article
- A comparison of diagnostic methods for canine Ehrlichiosis: Microscopy and RNases hybridization-assisted amplification technology compared with the quantitative polymerase chain reaction.Veterinary world · 2025Article
- Molecular detection of Ehrlichia Sp. in bat-associated chigger mites (trombidiformes: leeuwenhoekiidae and trombiculidae S. s.) from Brazil.Experimental & applied acarology · 2025Article
- Visual Detection of Canine Monocytic Ehrlichiosis Using Polymerase Chain Reaction-Based Lateral Flow Biosensors.Animals : an open access journal from MDPI · 2025Article
- Ehrlichia sp. in dairy cattle from Bahia, Brazil: high seropositivity rates and molecular confirmation of Ehrlichia minasensis.BMC veterinary research · 2024Article
- Feline Vector-Borne Diseases and Their Possible Association with Hematological Abnormalities in Cats from Midwestern Brazil.Microorganisms · 2024Article
- Potential zoonotic role of the tick Amblyomma cf. oblongoguttatum (Ixodida: Ixodidae) in the bacterial transmission of Ehrlichia chaffeensis (Rickettsiales: Anaplasmataceae) in a deciduous tropical forest in Mexico.Journal of medical entomology · 2024Article
- Novel Tick-Borne Anaplasmataceae Genotypes in Tropical Birds from the Brazilian Pantanal Wetland.Microorganisms · 2024Article
- Molecular screening of tick-borne microorganisms in ticks from rural areas of Panama, with the first record of Ehrlichia minasensis in Rhipicephalus microplus from Central America.Veterinary research communications · 2024Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ehrlichia species are the etiological agents of emerging and life-threatening tick-borne human zoonoses, in addition to causing serious and fatal infections in companion animals and livestock. We developed the first tricolor TaqMan real-time polymerase chain reaction assay capable of simultaneously detecting and discriminating medically important ehrlichiae in a single reaction. Analytical sensitivity of 50 copies per reaction was attained with templates from Ehrlichia chaffeensis, Ehrlichia ewingii, and Ehrlichia canis by amplifying the genus-specific disulfide bond formation protein gene (dsb). Ehrlichia genus-specific dsb primers amplified DNA from all known Ehrlichia species but not from other rickettsial organisms including Anaplasma platys, Anaplasma phagocytophilum, Rickettsia conorii, or Rickettsia typhi. High species specificity was attained as each species-specific TaqMan probe (E. chaffeensis, E. ewingii, and E. canis) identified homologous templates but did not cross-hybridize with heterologous Ehrlichia templates at concentrations as high as 10(8) copies. Identification of E. chaffeensis, E. ewingii, and E. canis from natural and experimental infections, previously confirmed by polymerase chain reaction and serological or microscopic evidence, demonstrated the comparable specificity and sensitivity of the dsb real-time assay. This assay provides a powerful tool for prospective medical diagnosis for human and canine ehrlichioses and for ecologic and epidemiological studies involving arthropod and mammalian hosts.
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