ArticleThe Journal of molecular diagnostics : JMD2005
BIOMED-2 multiplex immunoglobulin/T-cell receptor polymerase chain reaction protocols can reliably replace Southern blot analysis in routine clonality diagnostics.
Article in The Journal of molecular diagnostics : JMD, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 98 citations in OpenAlex.
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- Diagnostic Utility of Molecular Analysis in Cutaneous T-Cell Lymphoma: Tunisian Series on Clonality of TCRG Gene Rearrangement.South Asian journal of cancer · 2025Article
- Article
- Experience with the BIOMED-2 standardized polymerase chain reaction protocol for immunoglobulin and T- cell receptor clonality analysis in the Instituto Nacional de Cancerología, ColombiaBiomedica : revista del Instituto Nacional de Salud · 2022Article
- Combined detection of lymphocyte clonality and MALT1 translocations in bronchoalveolar lavage fluid for diagnosing pulmonary lymphomas.Scientific reports · 2021Article
- Evaluation of a worldwide EQA scheme for complex clonality analysis of clinical lymphoproliferative cases demonstrates a learning effect.Virchows Archiv : an international journal of pathology · 2021Article
- Detection of immunoglobulin and T-cell receptor gene rearrangements in angioimmunoblastic T-cell lymphoma.International journal of clinical and experimental pathology · 2018Article
- The value of detecting immunoglobulin gene rearrangements in the diagnosis of B-cell lymphoma.Oncotarget · 2017Article
- Utility of an immunoglobulin gene rearrangement assay based on multiplex PCR in detecting bone marrow involvement in B-cell non-Hodgkin lymphoma.Blood research · 2017Article
- Idiopathic hypereosinophilia is clonal disorder? Clonality identified by targeted sequencing.PloS one · 2017Article
- Methodologic Considerations in the Application of Next-Generation Sequencing of Human TRB Repertoires for Clinical Use.The Journal of molecular diagnostics : JMD · 2017Article
- Fluorine-18 fluorodeoxyglucose positron emission tomography/computed tomography evaluation of subcutaneous panniculitis-like T cell lymphoma and treatment response.World journal of clinical cases · 2016Article
- Hemophagocytic Lymphohistiocytosis in Children: Pathogenesis and Treatment.Frontiers in pediatrics · 2016Review
- Increased expression of interferon signaling genes in the bone marrow microenvironment of myelodysplastic syndromes.PloS one · 2015Article
- Clonality analysis of lymphoid proliferations using the BIOMED-2 clonality assays: a single institution experience.Radiology and oncology · 2014Article
- Infectious mononucleosis accompanied by clonal proliferation of EBV-infected cells and infection of CD8-positive cells.International journal of hematology · 2014Article
- Network properties derived from deep sequencing of human B-cell receptor repertoires delineate B-cell populations.Genome research · 2013Article
- Dysregulated RasGRP1 responds to cytokine receptor input in T cell leukemogenesis.Science signaling · 2013Article
- Analysis of immunoglobulin and T cell receptor gene rearrangement in the bone marrow of lymphoid neoplasia using BIOMED-2 multiplex polymerase chain reaction.International journal of medical sciences · 2013Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To establish the most sensitive and efficient strategy of clonality diagnostics via immunoglobulin and T-cell receptor gene rearrangement studies in suspected lymphoproliferative disorders, we evaluated 300 samples (from 218 patients) submitted consecutively for routine diagnostics. All samples were studied using the BIOMED-2 multiplex polymerase chain reaction (PCR) protocol. In 176 samples, Southern blot (SB) data were also available, and the two types of molecular results were compared. Results of PCR and SB analysis of both T-cell receptor and immunoglobulin loci were concordant in 85% of samples. For discordant results, PCR results were more consistent with the final diagnosis in 73% of samples. No false-negative results were obtained by PCR analysis. In contrast, SB analysis failed to detect clonality in a relatively high number of samples, mainly in cases of low tumor burden. We conclude that the novel BIOMED-2 multiplex PCR strategy is of great value in diagnosing patients with suspected B- and T-cell proliferations. Because of its higher speed, efficiency, and sensitivity, it can reliably replace SB analysis in clonality diagnostics in a routine laboratory setting. Just as with SB results, PCR results should always be interpreted in the context of clinical, immunophenotypical, and histopathological data.
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