ArticleThe Journal of molecular diagnostics : JMD2005
A multiplex polymerase chain reaction microarray assay to detect bioterror pathogens in blood.
Article in The Journal of molecular diagnostics : JMD, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Multiplex detection and identification of viral, bacterial, and protozoan pathogens in human blood and plasma using an expanded high-density resequencing microarray platform.Frontiers in molecular biosciences · 2024Article
- Comparison of multiplex PCR hybridization-based and singleplex real-time PCR-based assays for detection of low prevalence pathogens in spiked samples.Journal of microbiological methods · 2017Article
- Customizable PCR-microplate array for differential identification of multiple pathogens.Journal of food protection · 2013Article
- Reverse line blot macroarray for simultaneous detection and characterization of four biological warfare agents.Indian journal of microbiology · 2013Article
- Article
- A New Generation Microarray for the Simultaneous Detection and Identification of Yersinia pestis and Bacillus anthracis in Food.Journal of pathogens · 2012Article
- Reliable detection of Bacillus anthracis, Francisella tularensis and Yersinia pestis by using multiplex qPCR including internal controls for nucleic acid extraction and amplification.BMC microbiology · 2010Article
- Detection of blood-transmissible agents: can screening be miniaturized?Transfusion · 2010Review
- [Microbiological diagnosis: Etiologic driven and syndrome driven].Antibiotiques (Paris, France : 1999) · 2009Article
- Large scale multiplex PCR improves pathogen detection by DNA microarrays.BMC microbiology · 2009Article
- Applications of microarrays in pathogen detection and biodefence.Trends in biotechnology · 2009Review
- Multiplexed identification of blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary electrophoresis assay.Journal of clinical microbiology · 2007Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heightened concern about the dangers of bioterrorism requires that measures be developed to ensure the safety of the blood supply. Multiplex detection of such agents using a blood-screening DNA microarray is a sensitive and specific method to screen simultaneously for a number of suspected agents. We have developed and optimized a multiplex polymerase chain reaction microarray assay to screen blood for three potential bioterror bacterial pathogens and a human ribosomal RNA gene internal control. The analytical sensitivity of the assay was demonstrated to be 50 colony-forming units/ml for Bacillus anthracis, Francisella tularensis, and Yersinia pseudotuberculosis (surrogate for Yersinia pestis). The absence of any false-positives demonstrated high analytical specificity. Screening B. anthracis-infected mouse blood samples and uninfected controls demonstrated effectiveness and specificity in a preclinical application. This study represents proof of the concept of microarray technology to screen simultaneously for multiple bioterror pathogens in blood samples.
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