ArticleNucleic acids research2005
Atomic force microscopy shows that vaccinia topoisomerase IB generates filaments on DNA in a cooperative fashion.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Exploring protein-mediated compaction of DNA by coarse-grained simulations and unsupervised learning.Biophysical journal · 2024Article
- Transcriptional repression by a secondary DNA binding surface of DNA topoisomerase I safeguards against hypertranscription.Nature communications · 2023Article
- The mismatch repair endonuclease MutLα tethers duplex regions of DNA together and relieves DNA torsional tension.Nucleic acids research · 2023Article
- Article
- Expression and Purification of Vaccinia Virus DNA Topoisomerase IB Produced in the Silkworm-Baculovirus Expression System.Molecular biotechnology · 2019Article
- Studying protein-DNA interactions using atomic force microscopy.Seminars in cell & developmental biology · 2018Review
- The mismatch repair and meiotic recombination endonuclease Mlh1-Mlh3 is activated by polymer formation and can cleave DNA substrates in trans.PLoS biology · 2017Article
- Importance of disentanglement and entanglement during DNA replication and segregation: Comment on: "Disentangling DNA molecules" by Alexander Vologodskii.Physics of life reviews · 2016Article
- A robust assay to measure DNA topology-dependent protein binding affinity.Nucleic acids research · 2015Article
- Simulations of DNA topoisomerase 1B bound to supercoiled DNA reveal changes in the flexibility pattern of the enzyme and a secondary protein-DNA binding site.Nucleic acids research · 2014Article
- Variola type IB DNA topoisomerase: DNA binding and supercoil unwinding using engineered DNA minicircles.Biochemistry · 2014Article
- A highly processive topoisomerase I: studies at the single-molecule level.Nucleic acids research · 2014Article
- Crystal structure of a bacterial topoisomerase IB in complex with DNA reveals a secondary DNA binding site.Structure (London, England : 1993) · 2010Article
- Topoisomerase IB-DNA interactions: X marks the spot.Structure (London, England : 1993) · 2010Article
- Mutational analysis of the preferential binding of human topoisomerase I to supercoiled DNA.The FEBS journal · 2009Article
- Monte carlo simulations of protein assembly, disassembly, and linear motion on DNA.Biophysical journal · 2008Article
- Single-molecule observations of topotecan-mediated TopIB activity at a unique DNA sequence.Nucleic acids research · 2008Article
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Authors and funding
6 authors.
Funding
Abstract
Type IB DNA topoisomerases cleave and rejoin one strand of the DNA duplex, allowing for the removal of supercoils generated during replication and transcription. In addition, electron microscopy of cellular and viral TopIB-DNA complexes has suggested that the enzyme promotes long-range DNA-DNA crossovers and synapses. Here, we have used the atomic force microscope to visualize and quantify the interaction between vaccinia topoisomerase IB (vTopIB) and DNA. vTopIB was found to form filaments on nicked-circular DNA by intramolecular synapsis of two segments of a single DNA molecule. Measuring the filament length as a function of protein concentration showed that synapsis is a highly cooperative process. At high protein:DNA ratios, synapses between distinct DNA molecules were observed, which led to the formation of large vTopIB-induced DNA clusters. These clusters were observed in the presence of Mg2+, Ca2+ or Mn2+, suggesting that the formation of intermolecular vTopIB-mediated DNA synapsis is favored by screening of the DNA charge.
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