ArticleNucleic acids research2005
Proximity ligation assays with peptide conjugate 'burrs' for the sensitive detection of spores.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 35 citations in OpenAlex.
- Implications of limits of detection of various methods for Bacillus anthracis in computing risks to human health.Applied and environmental microbiology · 2009Pooled it
- Combining Deep Phenotyping of Serum Proteomics and Clinical Data via Machine Learning for COVID-19 Biomarker Discovery.International journal of molecular sciences · 2022Article
- Proximity ligation assay: an ultrasensitive method for protein quantification and its applications in pathogen detection.Applied microbiology and biotechnology · 2021Review
- DNAzyme-Functionalized R-Phycoerythrin as a Cost-Effective and Environment-Friendly Fluorescent Biosensor for Aqueous PbSensors (Basel, Switzerland) · 2019Article
- Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Proximity assays for sensitive quantification of proteins.Biomolecular detection and quantification · 2015Review
- Phage display--a powerful technique for immunotherapy: 2. Vaccine delivery.Human vaccines & immunotherapeutics · 2012Review
- Rational, modular adaptation of enzyme-free DNA circuits to multiple detection methods.Nucleic acids research · 2011Article
- Diffractometric detection of proteins using microbead-based rolling circle amplification.Analytical chemistry · 2010Article
- Real-time PCR detection of protein analytes with conformation-switching aptamers.Analytical biochemistry · 2008Article
- Identification and characterization of Bacillus anthracis spores by multiparameter flow cytometry.Applied and environmental microbiology · 2008Article
- Deoxyribozymes that recode sequence information.Nucleic acids research · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The proximity ligation assay (PLA) has previously been used for the sensitive and specific detection of single proteins. In order to adapt PLA methods for the detection of cell surfaces, we have generated multivalent peptide-oligonucleotide-phycoerythrin conjugates ('burrs') that can bind adjacent to one another on a cell surface and be ligated together to form unique amplicons. Real-time PCR detection of burr ligation events specifically identified as few as 100 Bacillus anthracis, 10 Bacillus subtilis and 1 Bacillus cereus spore. Burrs should prove to be generally useful for detecting and mapping interactions and distances between cell surface proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.