ArticleJournal of bacteriology2005
NspS, a predicted polyamine sensor, mediates activation of Vibrio cholerae biofilm formation by norspermidine.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.
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Who cites it
113 citing papers in PubMed, 190 citations in OpenAlex.
- Elucidation of D-amino acid metabolic activity of ornithine decarboxylase from the hyperthermophile Thermotoga maritima.Extremophiles : life under extreme conditions · 2026Article
- Recycling of Undigested Proteins Provided by the Host to the Large Intestine Microbiota: Implication for Intestinal Bacterial Anabolism, Growth, and Physiology.Microorganisms · 2025Review
- The influence of the polyamine synthesis pathways onMicrobiology (Reading, England) · 2025Article
- Squalamine and claramine A1 disperseBiofilm · 2025Article
- Review
- Choline metabolism modulates cyclic-di-GMP signaling and virulence of Pseudomonas aeruginosa in a macrophage infection model.BMC infectious diseases · 2024Article
- Biofilm dispersal patterns revealed using far-red fluorogenic probes.PLoS biology · 2024Article
- A hybrid biosynthetic-catabolic pathway for norspermidine production.The Biochemical journal · 2024Article
- Biofilm dispersal patterns revealed using far-red fluorogenic probes.bioRxiv : the preprint server for biology · 2024Article
- Control of biofilm formation by anProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Characterization of an Arginine Decarboxylase fromBiomolecules · 2024Article
- Cell-lysis sensing drives biofilm formation in Vibrio cholerae.Nature communications · 2024Article
- Polyamine signaling communications play a key role in regulating the pathogenicity ofMicrobiology spectrum · 2023Article
- Control of Biofilm Formation by anbioRxiv : the preprint server for biology · 2023Article
- A bibliography study of Shewanella oneidensis biofilm.FEMS microbiology ecology · 2023Article
- Biotin Deficiency Induces Intestinal Dysbiosis Associated with an Inflammatory Bowel Disease-like Phenotype.Nutrients · 2023Article
- TheMicrobiology (Reading, England) · 2023Article
- Synergy between c-di-GMP and Quorum-Sensing Signaling in Vibrio cholerae Biofilm Morphogenesis.Journal of bacteriology · 2022Article
- Still rocking in the structural era: A molecular overview of the small multidrug resistance (SMR) transporter family.The Journal of biological chemistry · 2022Review
- Polyamine and Ethanolamine Metabolism in Bacteria as an Important Component of Nitrogen Assimilation for Survival and Pathogenicity.Medical sciences (Basel, Switzerland) · 2022Review
53 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Vibrio cholerae is both an environmental bacterium and a human intestinal pathogen. The attachment of bacteria to surfaces in biofilms is thought to be an important feature of the survival of this bacterium both in the environment and within the human host. Biofilm formation occurs when cell-surface and cell-cell contacts are formed to make a three-dimensional structure characterized by pillars of bacteria interspersed with water channels. In monosaccharide-rich conditions, the formation of the V. cholerae biofilm requires synthesis of the VPS exopolysaccharide. MbaA (locus VC0703), an integral membrane protein containing a periplasmic domain as well as cytoplasmic GGDEF and EAL domains, has been previously identified as a repressor of V. cholerae biofilm formation. In this work, we have studied the role of the protein NspS (locus VC0704) in V. cholerae biofilm development. This protein is homologous to PotD, a periplasmic spermidine-binding protein of Escherichia coli. We show that the deletion of nspS decreases biofilm development and transcription of exopolysaccharide synthesis genes. Furthermore, we demonstrate that the polyamine norspermidine activates V. cholerae biofilm formation in an MbaA- and NspS-dependent manner. Based on these results, we propose that the interaction of the norspermidine-NspS complex with the periplasmic portion of MbaA diminishes the ability of MbaA to inhibit V. cholerae biofilm formation. Norspermidine has been detected in bacteria, archaea, plants, and bivalves. We suggest that norspermidine serves as an intercellular signaling molecule that mediates the attachment of V. cholerae to the biotic surfaces presented by one or more of these organisms.
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