ArticleMolecular biology of the cell2006
The CLIP-170 homologue Bik1p promotes the phosphorylation and asymmetric localization of Kar9p.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Actin- and microtubule-based motors contribute to clathrin-independent endocytosis in yeast.Molecular biology of the cell · 2023Article
- Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast.Molecular biology of the cell · 2023Article
- Multivalency ensures persistence of a +TIP body at specialized microtubule ends.Nature cell biology · 2023Article
- The microtubule plus-end tracking protein Bik1 is required for chromosome congression.Molecular biology of the cell · 2022Article
- The microtubule-associated protein She1 coordinates directional spindle positioning by spatially restricting dynein activity.Journal of cell science · 2021Article
- Article
- Asymmetric cell division and replicative aging: a new perspective from the spindle poles.Current genetics · 2020Review
- Asymmetric inheritance of spindle microtubule-organizing centres preserves replicative lifespan.Nature cell biology · 2019Article
- Spindle pole power in health and disease.Current genetics · 2019Review
- Nuclear movement in fungi.Seminars in cell & developmental biology · 2018Review
- The TOG protein Stu2/XMAP215 interacts covalently and noncovalently with SUMO.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Mechanism of nuclear movements in a multinucleated cell.Molecular biology of the cell · 2017Article
- Kar9 controls the nucleocytoplasmic distribution of yeast EB1.Cell cycle (Georgetown, Tex.) · 2016Article
- Molecular basis of Kar9-Bim1 complex function during mating and spindle positioning.Molecular biology of the cell · 2016Article
- Emerging roles of sumoylation in the regulation of actin, microtubules, intermediate filaments, and septins.Cytoskeleton (Hoboken, N.J.) · 2015Review
- Improved Plasmids for Fluorescent Protein Tagging of Microtubules in Saccharomyces cerevisiae.Traffic (Copenhagen, Denmark) · 2015Article
- The Ubp15 deubiquitinase promotes timely entry into S phase in Saccharomyces cerevisiae.Molecular biology of the cell · 2015Article
- Electron tomography reveals novel microtubule lattice and microtubule organizing centre defects in +TIP mutants.PloS one · 2013Article
- The yeast homologue of the microtubule-associated protein Lis1 interacts with the sumoylation machinery and a SUMO-targeted ubiquitin ligase.Molecular biology of the cell · 2012Article
- The MEN mediates the effects of the spindle assembly checkpoint on Kar9-dependent spindle pole body inheritance in budding yeast.Cell cycle (Georgetown, Tex.) · 2012Article
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Authors and funding
3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Accurate positioning of the mitotic spindle in Saccharomyces cerevisiae is coordinated with the asymmetry of the two poles and requires the microtubule-to-actin linker Kar9p. The asymmetric localization of Kar9p to one spindle pole body (SPB) and microtubule (MT) plus ends requires Cdc28p. Here, we show that the CLIP-170 homologue Bik1p binds directly to Kar9p. In the absence of Bik1p, Kar9p localization is not restricted to the daughter-bound SPB, but it is instead found on both SPBs. Kar9p is hypophosphorylated in bik1delta mutants, and Bik1p binds to both phosphorylated and unphosphorylated isoforms of Kar9p. Furthermore, the two-hybrid interaction between full-length KAR9 and the cyclin CLB5 requires BIK1. The binding site of Clb5p on Kar9p maps to a short region within the basic domain of Kar9p that contains a conserved phosphorylation site, serine 496. Consistent with this, Kar9p is found on both SPBs in clb5delta mutants at a frequency comparable with that seen in kar9-S496A strains. Together, these data suggest that Bik1p promotes the phosphorylation of Kar9p on serine 496, which affects its asymmetric localization to one SPB and associated cytoplasmic MTs. These findings provide further insight into a mechanism for directing centrosomal inheritance.
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