ArticleMolecular biology of the cell2006
Cyclic GMP-specific phosphodiesterase-5 regulates motility of sea urchin spermatozoa.
Article in Molecular biology of the cell, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 39 citations in OpenAlex.
- Review
- Modular analysis of the control of flagellar Ca2+-spike trains produced by CatSper and CaV channels in sea urchin sperm.PLoS computational biology · 2020Article
- Absolute proteomic quantification reveals design principles of sperm flagellar chemosensation.The EMBO journal · 2020Article
- A coordinated sequence of distinct flagellar waveforms enables a sharp flagellar turn mediated by squid sperm pH-taxis.Scientific reports · 2017Article
- Sperm Sensory Signaling.Cold Spring Harbor perspectives in biology · 2017Review
- Adverse effects of common medications on male fertility.Nature reviews. Urology · 2015Review
- Soluble adenylyl cyclase of sea urchin spermatozoa.Biochimica et biophysica acta · 2014Review
- Intracellular pH in sperm physiology.Biochemical and biophysical research communications · 2014Review
- Ca(2+)-modulated ROS-GC1 transduction system in testes and its presence in the spermatogenic cells.Frontiers in molecular neuroscience · 2014Article
- 100 years of sperm chemotaxis.The Journal of general physiology · 2012Article
- What is the core oscillator in the speract-activated pathway of the Strongylocentrotus purpuratus sperm flagellum?Biophysical journal · 2012Article
- A unique 2-sulfated {beta}-galactan from the egg jelly of the sea urchin Glyptocidaris crenularis: conformation flexibility versus induction of the sperm acrosome reaction.The Journal of biological chemistry · 2009Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Motility, chemotaxis, and the acrosome reaction of animal sperm are all regulated by cyclic nucleotides and protein phosphorylation. One of the cyclic AMP-dependent protein kinase (PKA) substrates in sea urchin sperm is a member of the phosphodiesterase (PDE) family. The molecular identity and in vivo function of this PDE remained unknown. Here we cloned and characterized this sea urchin sperm PDE (suPDE5), which is an ortholog of human PDE5. The recombinant catalytic domain of suPDE5 hydrolyzes only cyclic GMP (cGMP) and the activity is pH-dependent. Phospho-suPDE5 localizes mainly to sperm flagella and the phosphorylation increases when sperm contact the jelly layer surrounding eggs. In vitro dephosphorylation of suPDE5 decreases its activity by approximately 50%. PDE5 inhibitors such as Viagra block the activity of suPDE5 and increase sperm motility. This is the first PDE5 protein to be discovered in animal sperm. The data are consistent with the hypothesis that suPDE5 regulates cGMP levels in sperm, which in turn modulate sperm motility.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.