ArticleThe Journal of biological chemistry2005
Two-dimensional kinetics regulation of alphaLbeta2-ICAM-1 interaction by conformational changes of the alphaL-inserted domain.
Article in The Journal of biological chemistry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 72 citations in OpenAlex.
- Structure, function, and immunomodulation of the CD8 co-receptor.Frontiers in immunology · 2024Review
- Biomembrane force probe (BFP): Design, advancements, and recent applications to live-cell mechanobiology.Exploration (Beijing, China) · 2023Review
- Machine learning/molecular dynamic protein structure prediction approach to investigate the protein conformational ensemble.Scientific reports · 2022Article
- Immune-mediated alopecias and their mechanobiological aspects.Cells & development · 2022Review
- Neuromechanobiology: An Expanding Field Driven by the Force of Greater Focus.Advanced healthcare materials · 2021Review
- The kinetics of E-selectin- and P-selectin-induced intermediate activation of integrin αLβ2 on neutrophils.Journal of cell science · 2021Article
- Single-molecule investigations of T-cell activation.Current opinion in biomedical engineering · 2019Article
- Cadherin Extracellular Domain Clustering in the Absence ofThe journal of physical chemistry letters · 2019Article
- Ligand-specific binding forces of LFA-1 and Mac-1 in neutrophil adhesion and crawling.Molecular biology of the cell · 2018Article
- Article
- Kinetic Measurements Reveal Enhanced Protein-Protein Interactions at Intercellular Junctions.Scientific reports · 2016Article
- Immobilized IL-8 Triggers Phagocytosis and Dynamic Changes in Membrane Microtopology in Human Neutrophils.Annals of biomedical engineering · 2015Article
- α-catenin cytomechanics--role in cadherin-dependent adhesion and mechanotransduction.Journal of cell science · 2014Article
- Mechanical regulation of T-cell functions.Immunological reviews · 2013Review
- Biophysical description of multiple events contributing blood leukocyte arrest on endothelium.Frontiers in immunology · 2013Article
- Article
- Observing force-regulated conformational changes and ligand dissociation from a single integrin on cells.The Journal of cell biology · 2012Article
- Resolving two-dimensional kinetics of the integrin αIIbβ3-fibrinogen interactions using binding-unbinding correlation spectroscopy.The Journal of biological chemistry · 2012Article
- T cell antigen recognition at the cell membrane.Molecular immunology · 2012Review
- Cadherin-dependent mechanotransduction depends on ligand identity but not affinity.Journal of cell science · 2012Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The leukocyte integrin alphaLbeta2 mediates cell adhesion and migration during inflammatory and immune responses. Ligand binding of alphaLbeta2 is regulated by or induces conformational changes in the inserted (I) domain. By using a micropipette, we measured the conformational regulation of two-dimensional (2D) binding affinity and the kinetics of cell-bound intercellular adhesion molecule-1 interacting with alphaLbeta2 or isolated I domain expressed on K562 cells. Locking the I domain into open and intermediate conformations with a disulfide bond increased the affinities by approximately 8000- and approximately 30-fold, respectively, from the locked closed conformation, which has similar affinity as the wild-type I domain. Most surprisingly, the 2D affinity increases were due mostly to the 2D on-rate increases, as the 2D off-rates only decreased by severalfold. The wild-type alphaLbeta2, but not its I domain in isolation, could be up-regulated by Mn2+ or Mg2+ to have high affinities and on-rates. Locking the I domain in any of the three conformations abolished the ability of divalent cations to regulate 2D affinity. These results indicate that a downward displacement of the I domain C-terminal helix, induced by conformational changes of other domains of the alphaLbeta2, is required for affinity and on-rate up-regulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.