ArticleInternational journal of legal medicine2006
Low-volume amplification on chemically structured chips using the PowerPlex16 DNA amplification kit.
Article in International journal of legal medicine, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Naturally acquired microchimerism: implications for transplantation outcome and novel methodologies for detection.Chimerism · 2014Review
- Current genetic methodologies in the identification of disaster victims and in forensic analysis.Journal of applied genetics · 2012Review
- Article
- Forensic trace DNA: a review.Investigative genetics · 2010Article
- Laser capture microdissection in forensic research: a review.International journal of legal medicine · 2010Review
- Automatic retrieval of single microchimeric cells and verification of identity by on-chip multiplex PCR.Journal of cellular and molecular medicine · 2010Article
- Reduced-volume and low-volume typing of Y-chromosomal SNPs to obtain Finnish Y-chromosomal compound haplotypes.International journal of legal medicine · 2009Article
- Single lymphocytes from two healthy individuals with mitochondrial point heteroplasmy are mainly homoplasmic.International journal of legal medicine · 2008Article
- Low volume amplification and sequencing of mitochondrial DNA on a chemically structured chip.International journal of legal medicine · 2007Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In forensic DNA analysis, improvement of DNA typing technologies has always been an issue. It has been shown that DNA amplification in low volumes is a suitable way to enhance the sensitivity and efficiency of amplification. In this study, DNA amplification was performed on a flat, chemically structured glass slide in 1-microl reaction volumes from cell line DNA contents between 1,000 and 4 pg. On-chip DNA amplification reproducibly yielded full allelic profiles from as little as 32 pg of template DNA. Applicability on the simultaneous amplification of 15 short tandem repeats and of a segment of the Amelogenin gene, which are routinely used in forensic DNA analysis, is shown. The results are compared to conventional in-tube amplification carried out in 25-microl reaction volumes.
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Registered trials
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