ArticleMolecular genetics and genomics : MGG2005
Contribution of 5'- and 3'-untranslated regions of plastid mRNAs to the expression of Chlamydomonas reinhardtii chloroplast genes.
Article in Molecular genetics and genomics : MGG, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 104 citations in OpenAlex.
- Engineering bidirectional chloroplast promoters for tunable co-expression of multiple genes in microalgae (Chlamydomonas reinhardtii).Communications biology · 2026Article
- Expression of recombinant Cap antigen of porcine circovirus type 2 in the chloroplast ofFrontiers in plant science · 2025Article
- Heterologous Gene Expression in Chlamydomonas reinhardtii Chloroplast by Heterologous Promoters and Terminators, Intercistronic Expression Elements and Minichromosome.Microbial biotechnology · 2024Article
- A Chloroplast-Localised Fluorescent Protein Enhances the Photosynthetic Action Spectrum in Green Algae.Microorganisms · 2022Article
- Towards green biomanufacturing of high-value recombinant proteins using promising cell factory: Chlamydomonas reinhardtii chloroplast.Bioresources and bioprocessing · 2022Review
- Harnessing the Algal Chloroplast for Heterologous Protein Production.Microorganisms · 2022Review
- Retrograde and anterograde signaling in the crosstalk between chloroplast and nucleus.Frontiers in plant science · 2022Review
- Multigenic engineering of the chloroplast genome in the green algaMicrobiology (Reading, England) · 2020Article
- mCherry Protein as an In Vivo Quantitative Reporter of Gene Expression in the Chloroplast of Chlamydomonas reinhardtii.Molecular biotechnology · 2020Article
- Hosts for Hostile Protein Production: The Challenge of Recombinant Immunotoxin Expression.Biomedicines · 2019Review
- Recent Advances and Current Challenges in Synthetic Biology of the Plastid Genetic System and Metabolism.Plant physiology · 2019Review
- Manipulation of the microalgal chloroplast by genetic engineering for biotechnological utilization as a green biofactory.World journal of microbiology & biotechnology · 2018Review
- A downstream box fusion allows stable accumulation of a bacterial cellulase inBiotechnology for biofuels · 2018Article
- Comparison of effectiveness of 5'-regulatory sequences in transplastomic tobacco chloroplasts.Transgenic research · 2017Article
- Transgene Expression in Microalgae-From Tools to Applications.Frontiers in plant science · 2016Review
- Improving recombinant protein production in the Chlamydomonas reinhardtii chloroplast using vivid Verde Fluorescent Protein as a reporter.Biotechnology journal · 2015Article
- Establishing Chlamydomonas reinhardtii as an industrial biotechnology host.The Plant journal : for cell and molecular biology · 2015Review
- Article
- Photosynthetic gene expression in higher plants.Photosynthesis research · 2013Review
- Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives.Plant cell reports · 2012Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Expression of chloroplast genes is primarily regulated posttranscriptionally, and a number of RNA elements, found in either the 5'- or 3'-untranslated regions (UTRs) of plastid mRNAs, that impact gene expression have been identified. Complex regulatory and feedback mechanisms influence both translation and protein accumulation, making assignment of roles for specific RNA elements difficult. To identify specific contributions made by various UTRs on translation of plastid mRNAs, we used a heterologous gfp reporter gene that is fused combinatorially to chloroplast 5'- and 3'-UTRs. In general, the 5'-UTR, including the promoter, of the plastid atpA and psbD genes produced the highest levels of chimeric mRNA and protein accumulation, while the 5'-UTR of the rbcL and psbA genes produced less mRNA and protein. Varying the 3'-UTR had little impact on mRNA and protein accumulation, as long as a 3'-UTR was present. Overall, accumulation of chimeric mRNAs was proportional to protein accumulation, with a few notable exceptions. Light-regulated translation continues to operate in chimeric mRNAs containing the 5'-UTR of either the psbA or psbD mRNAs, despite translation of these two chimeric mRNAs at very different efficiencies, suggesting that translational efficiency and light-regulated translation are separate events. Translation of some chimeric mRNAs was much more efficient than others, suggesting that interactions between the untranslated and coding sequences can dramatically impact translational efficiency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.