ReviewGene therapy2005
Real-time quantitative PCR for the design of lentiviral vector analytical assays.
Review in Gene therapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Facilitating Gene Editing in Human Lymphoma Cells Using Murine Ecotropic γ-Retroviruses.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Strategy and Application of Viral Potency Assays in Cell and Gene Therapy.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Quantitation and integrity evaluation of RNA genome in lentiviral vectors by direct reverse transcription-droplet digital PCR (direct RT-ddPCR).Scientific reports · 2023Article
- Effect of inactivating RNA viruses by coupled UVC and UVA LEDs evaluated by a viral surrogate commonly used as a genetic vector.Biomedical optics express · 2022Article
- Identification of growth hormone receptor as a relevant target for precision medicine in low-EGFR expressing glioblastoma.Clinical and translational medicine · 2022Article
- A VHH-Based Anti-MUC1 Chimeric Antigen Receptor for Specific Retargeting of Human Primary T Cells to MUC1-Positive Cancer Cells.Cell journal · 2021Article
- Infectious titer determination of lentiviral vectors using a temporal immunological real-time imaging approach.PloS one · 2021Article
- Rapid In-Process Monitoring of Lentiviral Vector Particles by High-Performance Liquid Chromatography.Molecular therapy. Methods & clinical development · 2020Article
- Super-rapid quantitation of the production of HIV-1 harboring a luminescent peptide tag.The Journal of biological chemistry · 2020Article
- GlutathioneScience signaling · 2019Article
- A Lentiviral Vector Allowing Physiologically Regulated Membrane-anchored and Secreted Antibody Expression Depending on B-cell Maturation Status.Molecular therapy : the journal of the American Society of Gene Therapy · 2015Article
- Universal real-time PCR-based assay for lentiviral titration.Molecular biotechnology · 2015Article
- Rapid titration of retroviral vectors using a β-lactamase protein fragment complementation assay.Gene therapy · 2013Article
- Article
- Article
- Highly efficient concentration of lenti- and retroviral vector preparations by membrane adsorbers and ultrafiltration.BMC biotechnology · 2011Article
- Changes in the accessibility of the HIV-1 Integrase C-terminus in the presence of cellular proteins.Retrovirology · 2010Article
- Analysis of human immunodeficiency virus type 1 vector cis- and trans-acting elements production by means of Semliki Forest virus.Gene therapy · 2009Article
- Recombinant lentivector as a genetic immunization vehicle for antitumor immunity.Expert review of vaccines · 2007Review
- Gene delivery by lentivirus vectors.Molecular biotechnology · 2007Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
From the recent and emerging concerns for approving lentiviral vector-mediated gene transfer in human clinical applications, several analytical methods have been applied in preclinical models to address the lentiviral vector load in batches, cells or tissues. This review points out the oldest generation methods (blots, RT activity, standard PCR) as well as a full description of the newest real-time quantitative PCR (qPCR) applications. Combinations of primer and probe sequences, which have worked in the lentiviral amplification context, have been included in the effort to dress an exhaustive list. Also, great variations have been observed from interlaboratory results, we have tempted to compare between them the different analytical methods that have been used to consider (i) the titration of lentiviral vector batches, (ii) the absence of the susceptible emerging replicative lentiviruses or (iii) the lentiviral vector biodistribution in the organism.
Indexed as
Identifiers
16231054What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.