ArticleProceedings of the National Academy of Sciences of the United States of America2005
Synergistic action of E74B and ecdysteroid receptor in activating a 20-hydroxyecdysone effector gene.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- 20-hydroxyecdysone-mediated regulation of the ribosomal protein Cs40SRPS4 via CsE74A is essential for vitellogenesis in Chilo suppressalis.Insect science · 2026Article
- The AaFoxA factor regulates female reproduction through chromatin remodeling in the mosquito vector Aedes aegypti.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- The ecdysone-induced bZIP transcription factor MafB establishes a positive feedback loop to enhance vitellogenesis and reproduction in theProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Ecdysone signaling mediates the trade-off between immunity and reproduction via suppression of amyloids in the mosquito Aedes aegypti.PLoS pathogens · 2022Article
- Direct and indirect gene repression by the ecdysone cascade during mosquito reproductive cycle.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Influence of hyperproteinemia on reproductive development in an invertebrate model.International journal of biological sciences · 2019Article
- HR38, an ortholog of NR4A family nuclear receptors, mediates 20-hydroxyecdysone regulation of carbohydrate metabolism during mosquito reproduction.Insect biochemistry and molecular biology · 2018Article
- Methoprene-tolerant (Met) and Krüpple-homologue 1 (Kr-h1) are required for ovariole development and egg maturation in the brown plant hopper.Scientific reports · 2015Article
- Interplay among Drosophila transcription factors Ets21c, Fos and Ftz-F1 drives JNK-mediated tumor malignancy.Disease models & mechanisms · 2015Article
- A comparative analysis of reproductive biology of insect vectors of human disease.Current opinion in insect science · 2015Article
- Temporal Coordination of Carbohydrate Metabolism during Mosquito Reproduction.PLoS genetics · 2015Article
- Juvenile hormone and its receptor, methoprene-tolerant, control the dynamics of mosquito gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Ecdysone-responsive transcription factors determine the expression region of target cuticular protein genes in the epidermis of Bombyx mori.Development genes and evolution · 2012Article
- Distinct roles of isoforms of the heme-liganded nuclear receptor E75, an insect ortholog of the vertebrate Rev-erb, in mosquito reproduction.Molecular and cellular endocrinology · 2012Article
- A critical role of the nuclear receptor HR3 in regulation of gonadotrophic cycles of the mosquito Aedes aegypti.PloS one · 2012Article
- Programmed autophagy in the fat body of Aedes aegypti is required to maintain egg maturation cycles.PloS one · 2011Article
- Profile of Alexander S. Raikhel.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Distinct roles of Broad isoforms in regulation of the 20-hydroxyecdysone effector gene, Vitellogenin, in the mosquito Aedes aegypti.Molecular and cellular endocrinology · 2007Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
A number of insect effector genes activated by the steroid hormone 20-hydroxyecdysone (20E) are dually controlled by the ecdysteroid receptor (EcR/USP) and products of ecdysteroid early responsive genes (E74, E75, and Broad). However, the molecular mechanism of this dual action is poorly understood. Here we examined transcriptional activation of the vitellogenin (Vg) gene in the yellow fever mosquito, Aedes aegypti, by EcR/USP and E74 in response to an elevation of 20E titers. There are two isoforms of the Aedes E74 gene, AaE74A and AaE74B, which have a common C-terminal Ets DNA-binding domain and isoform-specific N termini in the female mosquito. Inhibiting expression of AaE74B but not AaE74A by RNA interference led to substantial reduction in the Vg gene expression. AaE74B and the ecdysteroid receptor synergistically enhanced 20E-induced transcription of the Vg promoter. This action required the E74-binding sites and the ecdysone response elements in the Vg 5' regulatory region. Two-hybrid assays and coimmunoprecipitation analyses demonstrated direct interaction between AaE74B and AaEcR/AaUSP. Moreover, disruption of this interaction by a dominant negative E74 mutant abolished the enhanced activation of Vg. Therefore, the cooperative interaction between AaE74B and the ecdysteroid receptor is required for high-level expression of the Vg gene in vivo. The synergistic activation is accomplished through their 20E-dependent protein-protein interaction on the gene promoter. This study reveals how the 20E direct-indirect regulation of an effector gene is achieved at the molecular level.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.