ArticleGenes & development2005
Bem1p, a scaffold signaling protein, mediates cyclin-dependent control of vacuolar homeostasis in Saccharomyces cerevisiae.
Article in Genes & development, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Lipid flippases in polarized growth.Current genetics · 2021Review
- Cellular and metabolic engineering of oleaginous yeastEngineering in life sciences · 2019Review
- Cell-cycle control of cell polarity in yeast.The Journal of cell biology · 2019Article
- Regulation of small GTPase activity by G1 cyclins.Small GTPases · 2019Review
- Mapping the Synthetic Dosage Lethality Network ofG3 (Bethesda, Md.) · 2017Article
- Vtc5, a Novel Subunit of the Vacuolar Transporter Chaperone Complex, Regulates Polyphosphate Synthesis and Phosphate Homeostasis in Yeast.The Journal of biological chemistry · 2016Article
- Multiple metabolic requirements for size homeostasis and initiation of division inMicrobial cell (Graz, Austria) · 2014Article
- Morphogenesis and the cell cycle.Genetics · 2012Review
- Prion formation and polyglutamine aggregation are controlled by two classes of genes.PLoS genetics · 2011Article
- Phosphoinositide [PI(3,5)P2] lipid-dependent regulation of the general transcriptional regulator Tup1.Genes & development · 2011Article
- An overview of Cdk1-controlled targets and processes.Cell division · 2010Article
- p21-activated kinases Cla4 and Ste20 regulate vacuole inheritance in Saccharomyces cerevisiae.Eukaryotic cell · 2009Article
- Vacuole inheritance regulates cell size and branching frequency of Candida albicans hyphae.Molecular microbiology · 2009Article
- The Dcr2p phosphatase destabilizes Sic1p in Saccharomyces cerevisiae.Biochemical and biophysical research communications · 2007Article
- Phosphorylation of Rga2, a Cdc42 GAP, by CDK/Hgc1 is crucial for Candida albicans hyphal growth.The EMBO journal · 2007Article
- Ribosome biogenesis is sensed at the Start cell cycle checkpoint.Molecular biology of the cell · 2007Article
- Ccpg1, a novel scaffold protein that regulates the activity of the Rho guanine nucleotide exchange factor Dbs.Molecular and cellular biology · 2006Article
- Dcr2 targets Ire1 and downregulates the unfolded protein response in Saccharomyces cerevisiae.EMBO reports · 2006Article
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Authors and funding
6 authors.
Funding
Abstract
How proliferating cells maintain the copy number and overall size of their organelles is not clear. We had previously reported that in the budding yeast Saccharomyces cerevisiae the G1 cyclin Cln3p is required for vacuolar (lysosomal) homotypic fusion and loss of Cln3p leads to vacuolar fragmentation. The Cdc42p GTPase is also required for vacuole fusion. Here we show that the scaffold protein Bem1p, a critical regulator of Cdc42p activity, is a downstream effector of Cln3p and the cyclin-dependent kinase (Cdk) Cdc28p. Our results suggest that Bem1p is phosphorylated in a Cdk-dependent manner to promote vacuole fusion. Replacing Ser72 with Asp, to mimic phosphorylation at an optimal Cdk-consensus site located in the first SH3 domain of Bem1p, suppressed vacuolar fragmentation in cells lacking Cln3p. Using in vivo and in vitro assays, we found that Cln3p was unable to promote vacuole fusion in the absence of Bem1p or in the presence of a nonphosphorylatable Bem1p-Ser72Ala mutant. Furthermore, activation of Cdc42p also suppressed vacuolar fragmentation in the absence of Cln3p. Our results provide a mechanism that links cyclin-dependent kinase activity with vacuole fusion through Bem1p and the Cdc42p GTPase cycle.
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