ArticleProtoplasma2005
Redundant mechanisms for anaphase chromosome movements: crane-fly spermatocyte spindles normally use actin filaments but also can function without them.
Article in Protoplasma, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 28 citations in OpenAlex.
- Article
- Measurements of forces produced by the mitotic spindle using optical tweezers.Molecular biology of the cell · 2013Article
- Functional states of kinetochores revealed by laser microsurgery and fluorescent speckle microscopy.Molecular biology of the cell · 2011Article
- The molecular basis of anaphase A in animal cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- Do nuclear envelope and intranuclear proteins reorganize during mitosis to form an elastic, hydrogel-like spindle matrix?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Review
- The perpetual movements of anaphase.Cellular and molecular life sciences : CMLS · 2010Review
- Article
- Abundance of actin filaments in the preprophase band and mitotic spindle of brick1 Zea mays mutant.Protoplasma · 2009Article
- Asunder is a critical regulator of dynein-dynactin localization during Drosophila spermatogenesis.Molecular biology of the cell · 2009Article
- Mitosis: spindle evolution and the matrix model.Protoplasma · 2009Article
- What generates flux of tubulin in kinetochore microtubules?Protoplasma · 2008Article
- Phospholipase C and myosin light chain kinase inhibition define a common step in actin regulation during cytokinesis.BMC cell biology · 2007Article
- Calyculin A, an enhancer of myosin, speeds up anaphase chromosome movement.Cell & chromosome · 2007Article
- Actin and myosin inhibitors block elongation of kinetochore fibre stubs in metaphase crane-fly spermatocytes.Protoplasma · 2007Article
- Possible roles of actin and myosin during anaphase chromosome movements in locust spermatocytes.Protoplasma · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin inhibitors block or slow anaphase chromosome movements in crane-fly spermatocytes, but stopping of movement is only temporary; we assumed that cells adapt to loss of actin by switching to mechanism(s) involving only microtubules. To test this, we produced actin-filament-free spindles: we added latrunculin B during prometaphase, 9-80 min before anaphase, after which chromosomes generally moved normally during anaphase. We confirmed the absence of actin filaments by staining with fluorescent phalloidin and by showing that cytochalasin D had no effect on chromosome movement. Thus, actin filaments are involved in normal anaphase movements, but in vivo, spindles nonetheless can function normally without them. We tested whether chromosome movements in actin-filament-free spindles arise via microtubules by challenging such spindles with anti-myosin drugs. Y-27632 and BDM (2,3-butanedione monoxime), inhibitors that affect myosin at different regulatory levels, blocked chromosome movement in normal spindles and in actin-filament-free spindles. We tested whether BDM has side effects on microtubule motors. BDM had no effect on ciliary and sperm motility or on ATPase activity of isolated ciliary axonemes, and thus it does not directly block dynein. Nor does it block kinesin, assayed by a microtubule sliding assay. BDM could conceivably indirectly affect these microtubule motors, though it is unlikely that it would have the same side effect on the motors as Y-27632. Since BDM and Y-27632 both affect chromosome movement in the same way, it would seem that both affect spindle myosin; this suggests that spindle myosin interacts with kinetochore microtubules, either directly or via an intermediate component.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.