ArticlePhotosynthesis research2002
Mutations of basic arginine residue 334 in the D1 protein of Photosystem II lead to unusual S(2) state properties in Synechocystis sp. PCC 6803.
Article in Photosynthesis research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed, 20 citations in OpenAlex.
- D1-Asn-298 in photosystem II is involved in a hydrogen-bond network near the redox-active tyrosine YThe Journal of biological chemistry · 2017Article
- Photoproduction of hydrogen by sulfur-deprived C. reinhardtii mutants with impaired photosystem II photochemical activity.Photosynthesis research · 2007Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
The C-terminus region of the D1 protein of Photosystem II (PS II) is situated on the lumenal side of the complex and is likely to be involved in the coordination of the active site Mn atoms of the water oxidation complex (WOC). The strictly conserved arginine at position 334 (D1-334) was targeted for site-directed mutagenesis to explore the hypothesis that it is involved in the PS II extrinsic protein binding, chloride binding, or proton transfer. Although it was found that D1-R334 probably not essential for these functions, mutations at this position were found to uniquely alter the kinetics of S-state cycling in general and the properties of the S(2) state in particular. Substitutions of a glutamate (D1-R334E) and a valine (D1-R334V) for D1-R334 lead to an unusually stable (t (1/2) >30 min at room temp) S(2) state, but not S(3), as measured by double flash measurements on the bare platinum electrode. However, measurements of fluorescence decay in the presence of DCMU suggest the S(2) state is only modestly affected by the mutations. Possible reasons for these apparently contradictory results are discussed.
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Registered trials
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