ArticlePhotosynthesis research2004
Minimal Extent of Sequence Homology Required for Homologous Recombination at the psbA Locus in Chlamydomonas reinhardtii Chloroplasts using PCR-generated DNA Fragments.
Article in Photosynthesis research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 50 citations in OpenAlex.
- A modular high-throughput approach for advancing synthetic biology in the chloroplast of Chlamydomonas.Nature plants · 2025Article
- Four Large Indels in Barley Chloroplast Mutator (International journal of molecular sciences · 2025Article
- Heterologous Gene Expression in Chlamydomonas reinhardtii Chloroplast by Heterologous Promoters and Terminators, Intercistronic Expression Elements and Minichromosome.Microbial biotechnology · 2024Article
- Chloroplast Cell-Free Systems from Different Plant Species as a Rapid Prototyping Platform.ACS synthetic biology · 2024Article
- Chloroplast Genome Engineering: A Plausible Approach to Combat Chili Thrips and Other Agronomic Insect Pests of Crops.Plants (Basel, Switzerland) · 2023Review
- Three Parts of the Plant Genome: On the Way to Success in the Production of Recombinant Proteins.Plants (Basel, Switzerland) · 2022Review
- Harnessing the Algal Chloroplast for Heterologous Protein Production.Microorganisms · 2022Review
- Plastid Transformation: How Does it Work? Can it Be Applied to Crops? What Can it Offer?International journal of molecular sciences · 2020Review
- Article
- The rpl23 gene and pseudogene are hotspots of illegitimate recombination in barley chloroplast mutator seedlings.Scientific reports · 2019Article
- Targeting mutations to the plastidial psbA gene of Chlamydomonas reinhardtii without direct positive selection.Scientific reports · 2019Article
- Recent Advances and Current Challenges in Synthetic Biology of the Plastid Genetic System and Metabolism.Plant physiology · 2019Review
- Construction of Plastid Expression Vector and Development of Genetic Transformation System for the Seaweed Pyropia yezoensis.Marine biotechnology (New York, N.Y.) · 2017Article
- Article
- Article
- Structure-based design of novel Chlamydomonas reinhardtii D1-D2 photosynthetic proteins for herbicide monitoring.Protein science : a publication of the Protein Society · 2009Article
- Identification of a plastid intercistronic expression element (IEE) facilitating the expression of stable translatable monocistronic mRNAs from operons.The Plant journal : for cell and molecular biology · 2007Article
- MoCloro: an extension of the Chlamydomonas reinhardtii modular cloning toolkit for microalgal chloroplast engineering.Physiologia plantarumArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Del1 mutant of the green alga Chlamydomonas reinhardtii with a defined deletion in the chloroplast encoded psbA gene is unable to grow photoautotrophically. We show here that this mutant can be transformed with PCR-generated psbA fragments of varying length to yield photosynthetically growing colonies. PCR fragments need not be purified but can be directly precipitated from the amplification reaction onto tungsten particles, allowing fast and efficient mutagenesis experiments. Flanking regions bordering the deletion breakpoints have been systematically shortened from both sides. The shortest fragment giving rise to significant numbers of transformants contains about 50 bp upstream and 120 bp downstream of the deletion breakpoint. This technique greatly simplifies comprehensive structure-function analyses of the D1 protein in Chlamydomonas, but could perhaps be adapted to other chloroplast genes in this or other organisms as well.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.